PRPTOGA MICROALGAE CDMO SERVICES

- I-Microalgae Library

Microalgae Seed Supply

▪ I-PROTOGA Microalgae Library iye yalondoloza cishe izinhlobo eziyikhulu zolwelwe oluncane, kuhlanganise kodwa olungagcini nje ngo-Haematococcus Pluvialis, Chlorella sp., Dictyosphaerium sp., Scenedesmus sp. kanye ne-Synechocystis sp.. Zonke izimbewu ze-algae ziyahlanzwa futhi zigunyazwe njengama-microalgae athile angasetshenziswa ocwaningweni lwesayensi.

Ukuhlukaniswa kwe-Microalgae

▪ I-PROTOGA ingahlukanisa futhi ihlanze ama-microalgae emvelo emachibini, emifuleni, emaxhaphozini, angahlolwa ngezingcindezi ezahlukene (izinga lokushisa eliphezulu/eliphansi, elimnyama/elikhanyayo njll.). Amakhasimende ethu angaba ngabanikazi be-microalgae ehlanziwe futhi ehloliwe ukuze kwenziwe ucwaningo, amalungelo obunikazi, ukuthuthukiswa kwezohwebo.

Ukuzalanisa inguquko

▪ I-PROTOGA isungule uhlelo olusebenzayo lwe-ARTP lwe-microalgae mutagenesis, olufanele ikakhulukazi uhlobo oluthile oluvamile. I-PROTOGA futhi ingakha uhlelo olusha lwe-ARTP kanye nebhange eliguquguqukayo lapho kudingeka ama-microalgae athile.

- Esimeme

Uma kuqhathaniswa namafutha ezinhlanzi nokudla okusekelwe ezilwaneni, ama-microalgae ayasimeme futhi anobungani bemvelo. I-Microalgae izoba yizixazululo ezithembisayo zenkinga ekhona embonini yokudla, ukulima kanye nokufudumala komhlaba.

I-PROTOGA izibophezele ekuthuthukiseni ubuchwepheshe obusha obuncane obusheshisa ukuguqulwa kwezimboni embonini ye-microalgae, esiza ekudambiseni inkinga yokudla emhlabeni wonke, ukushoda kwamandla kanye nokungcoliswa kwemvelo. Sikholelwa ukuthi ama-microalgae angakhuthaza umhlaba omusha ukuthi abantu baphile ngendlela enempilo neluhlaza.

- Ukukhiqiza Ngokwezifiso

I-Microalgae Fermentation & Post-Processing

i.PROTOGA yakhiwe ngaphezu kwamamitha-skwele ayi-100 wesitshalo esisezingeni le-C ngokuhambisana ne-ISO Class7 ne-GMP, kanye negumbi elihlala njalo lokushisa nomswakama kanye nendawo ehlanzekile ngokuhambisana nezidingo zelayisensi yokukhiqiza ukudla, engenziwa ngendlela oyifisayo ngokuvumelana nekhasimende. izidingo.
ii.Sihlonyiswe ngama-fermenter ahlukile asebenza ngokunembile ububanzi obuyi-5L kuya ku-1000L, afaka isikali selebhu ekukhiqizeni isikali somshayeli.
iii.Post-processing ihlanganisa ukuqoqwa kwamaseli, ukomiswa, ukugaywa kwebhola nokunye.
iv.Izindawo zokuhlola namathuluzi afana ne-HPLC ne-GC enza ukuhlaziya umkhiqizo we-biomass, i-carotenoid, ama-fatty acids, i-organic carbon, i-nitrogen, i-phosphorus nezinye izinto.

- Ibhayoloji yamangqamuzana

I-Microalgal Plasmid Bank
▪ I-Microalgal Plasmid Bank ihlanganisa kodwa ingagcini nje kuma-plasmid oguquko ajwayelekile. I-Plasmid Bank ihlinzeka ngezinhlobonhlobo zamavekhtha afanelekile futhi asebenza kahle ezifundweni ezahlukahlukene.

Ukuthuthukiswa kwe-AI kokulandelana kweGene
▪ I-PROTOGA inohlelo lokuthuthukisa izakhi zofuzo ngokufunda kwe-AI. Isibonelo, ingakwazi ukuthuthukisa i-ORF ezakhini zofuzo zangaphandle, ibone ukulandelana kokuvezwa kwezinga eliphezulu, isize ukukhomba i-overexpress yofuzo.

Ukucindezeleka ngokweqile ku-Chlamydomonas reinhardtii
▪ I-PROTOGA's Chlamydomonas reinhardtii iklanywe njenge-microalgal chassis ye-expression yangaphandle yamaprotheni amakwe nge-HA, Strep noma i-GFP. Ngokwezidingo zakho, amaprotheni okuhlosiwe angavezwa nge-cytoplasm noma i-chloroplast.

I-Gene Knockout ku-Chlamydomonas reinhardtii
▪ Ithimba lezobuchwepheshe le-PROTOGA lakhe uhlelo lokuhlela lwe-Crispr/cas9 kanye ne-Crispr/cas12a e-Chlamydomonas reinhardtii, okuhlanganisa ukuklanywa kwe-gRNA, isifanekiso se-DNA yomxhasi, ukuhlanganiswa okuyinkimbinkimbi nezinye izici, eziqhuba ukukhishwa kwezakhi zofuzo kanye ne-mutagenesis eqondiswe kusayithi.