I-Polysaccharide evela ku-Chlorella (PFC), njenge-polysaccharide yemvelo, idonse ukunakwa okuningi kwezazi eminyakeni yamuva nje ngenxa yezinzuzo zayo zobuthi obuphansi, imiphumela engemihle ephansi, kanye nemiphumela ebanzi. Imisebenzi yayo ekwehliseni i-lipids yegazi, i-anti-tumor, anti-inflammatory, anti-Parkinson's, anti-aging, njll. iqinisekiswe ngaphambilini kwi-vitro kanye nokuhlolwa kwe-vivo. Kodwa-ke, lisekhona igebe ocwaningweni lwe-PFC njengemoduli yokuzivikela yomuntu.

Amaseli e-Dendritic (DCs) angamaseli anamandla akhethekile aveza ama-antigen emzimbeni womuntu. Inombolo yama-DC emzimbeni womuntu incane kakhulu, futhi imodeli yokungeniswa kwe-cytokine ephakathi kwe-vitro, okuyigazi legazi lomuntu le-mononuclear cell-derived DCs (moDCs), isetshenziswa kakhulu. Imodeli ye-DC eyenziwe nge-in vitro yabikwa okokuqala ngo-1992, okuwuhlelo lwesiko lendabuko lwama-DC. Ngokuvamile, kudinga ukutshalwa izinsuku ezingu-6-7. Amaseli omnkantsha wegundane angakhuliswa nge-granulocyte macrophage colony-stimulating factor (GM-CSF) kanye ne-interleukin (IL) -4 ukuze kutholwe ama-DC angavuthiwe (iqembu le-PBS). Ama-Cytokines engezwa njengezisusa ezivuthiwe futhi akhuliswe izinsuku ezingu-1-2 ukuze kutholwe ama-DC avuthiwe. Olunye ucwaningo lubike ukuthi amaseli e-CD14 + ahlanziwe ahlanjululwa nge-interferon - β (IFN - β) noma i-IL-4 izinsuku ezingu-5, bese ekhuliswa nge-tumor necrosis factor-a (TNF-a) izinsuku ezingu-2 ukuze athole ama-DC aphezulu. ukubonakaliswa kwe-CD11c ne-CD83, enamandla amakhulu okukhuthaza ukwanda kwamaseli e-allogeneic CD4+T nama-CD8+T cells. Ama-polysaccharides amaningi avela emithonjeni yemvelo anomsebenzi omuhle kakhulu wokuzivikela komzimba, njengama-polysaccharides avela kumakhowe e-shiitake, amakhowe e-split gill, amakhowe e-Yunzhi, nama-Poria cocos, asetshenziswe emisebenzini yomtholampilo. Angakwazi ukuthuthukisa ngempumelelo umsebenzi wokuzivikela komzimba, athuthukise ukuzivikela komzimba, futhi asebenze njengemithi yokwelapha ye-adjuvant yokwelashwa kwe-anti-tumor. Kodwa-ke, kunemibiko embalwa yocwaningo nge-PFC njengemoduli yokuzivikela yomuntu. Ngakho-ke, lesi sihloko senza ucwaningo lokuqala ngendima kanye nezindlela ezihlobene ze-PFC ekukhuthazeni ukuvuthwa kwama-moDCs, ukuze kuhlolwe amandla e-PFC njengemoduli yokuzivikela yemvelo.

Ngenxa yengxenye ephansi kakhulu yama-DC ezicutshini zomuntu kanye nokulondolozwa okuphezulu kwezinhlobo zezilwane phakathi kwamagundane e-DC kanye nama-DC abantu, ukuze kuxazululwe ubunzima bocwaningo obubangelwa ukukhiqizwa kwe-DC ephansi, amamodeli okungenisa e-in vitro ama-DC athathwe kumaseli egazi atholakala egazini le-mononuclear. ziye zafundwa, ezingathola ama-DC ane-immunogenicity enhle ngesikhathi esifushane. Ngakho-ke, lolu cwaningo lusebenzise indlela yendabuko yokufaka i-DCs yomuntu ku-vitro: i-co culturing rhGM CSF kanye ne-rhIL-4 in vitro, ukushintsha okuphakathi nsuku zonke, nokuthola ama-DC angavuthiwe ngosuku lwe-5th; Ngosuku lwe-6th, amanani alinganayo e-PBS, PFC, kanye ne-LPS angeziwe ngokuya ngokuqoqwa futhi akhuliswe amahora angama-24 njengenqubo yesiko lokunxenxa ama-DC atholakala kumaseli egazi legazi le-mononuclear.

Ama-Polysaccharides atholakala emikhiqizweni yemvelo anezinzuzo zobuthi obuphansi kanye nezindleko eziphansi njengama-immunostimulants. Ngemuva kokuhlolwa kokuqala, iqembu lethu locwaningo lithole ukuthi i-PFC ikhulisa kakhulu umaka ovuthiwe we-CD83 ebusweni bamaseli e-DC atholakala kumaseli e-DC enziwe nge-vitro. Imiphumela ye-cytometry egelezayo ibonise ukuthi ukungenelela kwe-PFC ekugxilweni kwe-10 μ g / mL amahora angu-24 kubangele ukubonakaliswa okuphezulu kwe-CD83 yomaka ovuthiwe ebusweni be-DCs, okubonisa ukuthi ama-DC angena esimweni esivuthiwe. Ngakho-ke, iqembu lethu locwaningo linqume uhlelo lokungeniswa kwe-in vitro nohlelo lokungenelela. I-CD83 iyi-biomarker ebalulekile evuthiwe ebusweni be-DCs, kuyilapho i-CD86 isebenza njenge-molecule ebalulekile evuselelayo ebusweni bama-DC, esebenza njengesignali yesibili yokuvula amaseli e-T. Ukuvezwa okuthuthukisiwe kwama-biomarker amabili e-CD83 ne-CD86 kukhombisa ukuthi i-PFC ikhuthaza ukuvuthwa kwama-DC asuselwa kuseli legazi lomuntu we-mononuclear, okuphakamisa ukuthi i-PFC inganyusa ngasikhathi sinye izinga lokuphuma kwama-cytokines ebusweni bama-DC. Ngakho-ke, lolu cwaningo luhlole amazinga ama-cytokines IL-6, TNF-a, ne-IL-10 afihliwe ama-DC esebenzisa i-ELISA. I-IL-10 ihlobene eduze nokubekezelelana kwe-immune ye-DCs, kanti ama-DC anokubekezela komzimba avame ukusetshenziswa ekwelapheni kwe-tumor, ehlinzeka ngemibono yokwelapha engase ibe khona yokubekezelelana kwamasosha omzimba ekufakweni kwesitho; Umndeni we-1L-6 udlala indima ebalulekile ekugomeni okungaphakathi nokuguquguqukayo, i-hematopoiesis, nemiphumela yokulwa nokuvuvukala; Kukhona izifundo ezibonisa ukuthi i-IL-6 ne-TGF β ngokuhlanganyela iqhaza ekuhlukaniseni amaseli e-Th17; Lapho umzimba uhlaselwa yigciwane, i-TNF-a ekhiqizwa ama-DCs ekuphenduleni ukusebenza kwegciwane isebenza njengento yokuvuthwa kwe-autocrine ukukhuthaza ukuvuthwa kwe-DC. Ukuvimbela i-TNF-a kuzobeka ama-DCs esigabeni esingavuthiwe, kuwavimbele ekusebenziseni ngokugcwele umsebenzi wabo wokwethulwa kwe-antigen. Idatha ye-ELISA kulolu cwaningo ibonise ukuthi izinga le-secretion ye-IL-10 eqenjini le-PFC lenyuke kakhulu uma kuqhathaniswa namanye amaqembu amabili, okubonisa ukuthi i-PFC ithuthukisa ukubekezelelana komzimba we-DCs; Amazinga akhulayo emfihlo we-IL-6 kanye ne-TNF-a aphakamisa ukuthi i-PFC ingase ibe nomthelela wokuthuthukisa i-DC ukuze ikhuthaze ukuhlukaniswa kwamaseli e-T.