Emikhakheni ehlukahlukene yocwaningo kanye nokusetshenziswa kwe-microalgae, ubuchwepheshe bokugcina isikhathi eside kwamaseli ama-microalgae bubalulekile. Izindlela zendabuko zokulondoloza ulwelwe oluncane lubhekana nezinselele eziningi, okuhlanganisa ukuncipha kokuzinza kwezakhi zofuzo, izindleko ezikhuphukile, kanye nezingozi zokungcola. Ukuze kubhekwane nalezi zinkinga, i-protoga isungule indlela ye-vitrification cryopreservation elungele ama-microalgae ahlukahlukene. Ukwakhiwa kwesisombululo se-cryopreservation kubalulekile ekugcineni amandla kanye nokuzinza kwezakhi zofuzo zamangqamuzana ama-microalgae.
Njengamanje, nakuba izicelo eziyimpumelelo zenziwe ku-Chlamydomonas reinhardtii, umehluko wesakhiwo somzimba namaselula phakathi kwezinhlobo zama-microalgae ahlukene kusho ukuthi i-microalgae ngayinye ingase idinge ukwakheka okuthile kwe-cryoprotectant. Uma kuqhathaniswa nezixazululo ze-cryopreservation ezisetshenziswa kwamanye amasu e-microbial kanye nesilwane se-cryopreservation, isixazululo se-cryopreservation se-microalgae sidinga ukucabangela ukwakheka kodonga lwamaseli, ukumelana nesithwathwa, kanye nokusabela okunobuthi okukhethekile kwezivikeli kumangqamuzana olwelwe oluyizinhlobo ezahlukene ze-algal.
Ubuchwepheshe be-vitrification cryopreservation of microalgae busebenzisa izixazululo ze-cryopreservation eziklanywe ngokukhethekile ukugcina amaseli emazingeni okushisa aphansi kakhulu, njenge-nitrogen ewuketshezi noma -80 ° C, ngemva kwenqubo yokupholisa ehleliwe. Amakristalu eqhwa ngokuvamile akheka ngaphakathi kwamaseli ngesikhathi sokupholisa, abangele ukulimala kwesakhiwo seseli nokulahlekelwa ukusebenza kwamangqamuzana, okuholela ekufeni kweseli. Ukuze kuthuthukiswe izixazululo ze-microalgae cryopreservation, i-protoga yenza ucwaningo olunzulu ngezici zamangqamuzana ama-microalgae, okuhlanganisa ukusabela kwawo kubavikeli abahlukahlukene kanye nendlela yokunciphisa ngempumelelo kakhulu umonakalo obangelwa ukuqhwa kanye nokucindezela kwe-osmotic. Lokhu kuhlanganisa ukulungiswa okuqhubekayo kohlobo, ukugxilisa ingqondo, ukulandelana kokwengezwa, ukupholisa kwangaphambili, nezinqubo zokuthola kabusha zama-ejenti avikelayo kusixazululo se-cryopreservation, okuphumela ekuthuthukisweni kwesisombululo esibanzi se-microalgae cryopreservation esibizwa ngokuthi i-Froznthrive ™ Kanye nobuchwepheshe bokufriza obusekela i-vitrification.
Isikhathi sokuthumela: Jul-19-2024