I-Polysaccharide evela kwi-Chlorella (PFC), njengepolysaccharide yendalo, itsalele ingqalelo enkulu kubaphengululi kwiminyaka yakutshanje ngenxa yeenzuzo zayo zetyhefu ephantsi, iziphumo ebezingalindelekanga eziphantsi, kunye neziphumo ezibanzi. Imisebenzi yayo ekunciphiseni i-lipids yegazi, i-anti-tumor, i-anti-inflammatory, anti-Parkinson's, i-anti-aging, njl. iye yaqinisekiswa kwangaphambili kwi-vitro kunye ne-vivo experiments. Nangona kunjalo, kusekho umsantsa kuphando kwi-PFC njengemodyuli yomzimba womntu.

Iiseli ze-Dendritic (i-DCs) zezona iiseli ezinamandla ezikhethekileyo ze-antigen-presententing kumzimba womntu. Inani le-DCs kumzimba womntu lincinci kakhulu, kwaye i-cytokine mediated in vitro model induction, ebizwa ngokuba yi-peripheral blood mononuclear cell-derived DCs (moDCs), isetyenziswa ngokuqhelekileyo. Imodeli ye-DC eyenziwe nge-in vitro yaqala ukuxelwa ngo-1992, eyona nkqubo yenkcubeko yemveli yee-DCs. Ngokuqhelekileyo, idinga ukulinywa kweentsuku ezi-6-7. Iiseli zomongo weMouse zinokukhuliswa kunye ne-granulocyte macrophage colony-stimulating factor (GM-CSF) kunye ne-interleukin (IL) -4 ukufumana i-DCs ekhulile (iqela le-PBS). I-Cytokines yongezwa njenge-stimuli ekhulile kwaye ikhuliswe kwiintsuku ze-1-2 ukufumana ii-DC ezivuthiweyo. Olunye uphando luchaze ukuba iiseli ze-CD14 + ezihlambulukileyo zomntu zikhuliswe nge-interferon - β (IFN - β) okanye i-IL-4 kwiintsuku ze-5, kwaye emva koko zikhuliswe nge-tumor necrosis factor-a (TNF-a) kwiintsuku ze-2 ukufumana i-DCs ephezulu. ukubonakaliswa kwe-CD11c kunye ne-CD83, enekhono elinamandla lokukhuthaza ukwanda kweeseli ze-allogeneic CD4 + T kunye neeseli ze-CD8 + T. Iipolysaccharides ezininzi ezivela kwimithombo yendalo zinomsebenzi ogqwesileyo we-immunomodulatory, onje nge-polysaccharides ukusuka kumakhowa e-shiitake, amakhowa aqhekezayo, amakhowa eYunzhi, kunye nePoria cocos, eziye zasetyenziswa kwiklinikhi. Banokuphucula ngokufanelekileyo umsebenzi wokuzikhusela komzimba, bakhulise ukhuseleko, kwaye basebenze njengonyango lwe-adjuvant kunyango lwe-anti-tumor. Nangona kunjalo, zimbalwa iingxelo zophando kwi-PFC njengemodyuli yomzimba womntu. Ngoko ke, eli nqaku liqhuba uphando lwangaphambili malunga nendima kunye neendlela ezinxulumene ne-PFC ekukhuthazeni ukuvuthwa kwee-moDCs, ukwenzela ukuvavanya amandla e-PFC njengemodyuli yendalo yomzimba.

Ngenxa yomlinganiselo ophantsi kakhulu we-DCs kwizihlunu zabantu kunye nolondolozo oluphezulu lweentlobo phakathi kwee-DCs ze-mouse kunye nee-DCs zabantu, ukuze kusonjululwe ubunzima bophando obubangelwa yimveliso ephantsi ye-DC, imifuziselo yokungeniswa kwe-in vitro ye-DCs ethathwe kwi-peripheral blood mononuclear cells. ziye zafundwa, ezinokuthi zifumane ii-DCs ezine-immunogenicity efanelekileyo ngexesha elifutshane. Ngoko ke, olu pho nonongo lusebenzise indlela yendabuko yokubangela i-DCs yabantu kwi-vitro: i-co culturing rhGM CSF kunye ne-rhIL-4 in vitro, ukutshintsha i-medium yonke imihla, kunye nokufumana i-DCs engakafiki ngosuku lwe-5; Ngosuku lwe-6, iivolumu ezilinganayo ze-PBS, i-PFC, kunye ne-LPS zongezwa ngokweqela kunye nenkcubeko yeeyure ze-24 njenge-protocol yenkcubeko yokunyathela i-DCs ephuma kwiiseli zegazi ze-mononuclear zegazi.

Iipolysaccharides ezivela kwiimveliso zendalo zineenzuzo zetyhefu ephantsi kunye nexabiso eliphantsi njenge-immunostimulants. Emva kovavanyo lokuqala, iqela lethu lophando lifumanise ukuba i-PFC yonyusa kakhulu i-CD83 eqolileyo kumphezulu wegazi lomntu we-mononuclear cell cell-derived DC cell eyenziwe kwi-vitro. Iziphumo ze-cytometry ezijikelezayo zibonise ukuba ukungenelela kwe-PFC kwi-concentration ye-10 μ g / mL kwiiyure ze-24 kubangele ukubonakaliswa okuphezulu kwe-CD83 ekhulile ebusweni be-DCs, ebonisa ukuba i-DCs ingene kwisimo esivuthiweyo. Ke ngoko, iqela lethu lophando limisele i-in vitro induction kunye nesicwangciso songenelelo. I-CD83 yibhayomarker ebalulekileyo eqolileyo kumphezulu we-DCs, ngelixa i-CD86 isebenza njengemolekyuli ebalulekileyo ye-co stimulatory kumphezulu we-DCs, isebenza njengophawu lwesibini lokuvula iiseli ze-T. Ukubonakaliswa okuphuculweyo kwee-biomarkers ezimbini ze-CD83 kunye ne-CD86 kubonisa ukuba i-PFC ikhuthaza ukuvuthwa kwegazi lomntu kwi-mononuclear cell-derived DCs, iphakamisa ukuba i-PFC inokunyusa ngaxeshanye inqanaba lokukhuselwa kwee-cytokines kumphezulu we-DCs. Ngoko ke, olu pho nonongo luvavanye amanqanaba e-cytokines IL-6, TNF-a, kunye ne-IL-10 efihliweyo yi-DCs esebenzisa i-ELISA. I-IL-10 inxulumene ngokusondeleyo nokunyamezela kwe-immune ye-DCs, kunye ne-DCs ezinokunyamezela komzimba zisetyenziswa ngokuqhelekileyo kunyango lwe-tumor, ukubonelela ngeengcamango zonyango ezinokuthi zivumelane nokunyamezela komzimba kwi-organ transplantation; Intsapho ye-1L-6 idlala indima ebalulekileyo ekukhuseleni okungaphakathi kunye nokuguquguqukayo, i-hematopoiesis, kunye nemiphumo echasayo; Kukho izifundo ezibonisa ukuba i-IL-6 kunye ne-TGF β ngokudibeneyo inxaxheba ekuhlukeni kweeseli ze-Th17; Xa umzimba uhlaselwa yintsholongwane, i-TNF-a eveliswa yi-DCs ekuphenduleni ukusebenza kwentsholongwane isebenza njengento yokuvuthwa kwe-autocrine ukukhuthaza ukuvuthwa kwe-DC. Ukuthintela i-TNF-a kuya kubeka ii-DCs kwisigaba esingenasiphelo, ukuthintela ukuba basebenzise ngokupheleleyo umsebenzi wabo wokubonisa i-antigen. Idatha ye-ELISA kolu phononongo ibonise ukuba inqanaba le-secretion ye-IL-10 kwiqela le-PFC liye lanyuka kakhulu xa kuthelekiswa namanye amaqela amabini, ebonisa ukuba i-PFC iphakamisa ukunyamezela komzimba kwi-DCs; Amanqanaba akhulayo e-secretion ye-IL-6 kunye ne-TNF-a acebisa ukuba i-PFC ingaba nefuthe lokuphucula i-DC ukukhuthaza ukuhlukana kweeseli ze-T.