Kwiinkalo ezahlukeneyo zophando kunye nokusetyenziswa kwe-microalgae, iteknoloji yokugcinwa kwexesha elide kweeseli ze-microalgae ibalulekile. Iindlela zemveli zokugcinwa kwe-microalgae zijongene nemingeni emininzi, kubandakanywa ukuhla kozinzo lwemfuza, ukunyuka kweendleko, kunye nokwanda kweengozi zongcoliseko. Ukujongana nale miba, iprotoga iye yaphuhlisa ubuchule be-vitrification cryopreservation efanelekileyo kwii-microalgae ezahlukeneyo. Ukuqulunqwa kwesisombululo se-cryopreservation kubalulekile ekugcineni amandla kunye nokuzinza kwemfuza yeeseli ze-microalgae.

Okwangoku, nangona izicelo eziphumeleleyo zenziwe kwi-Chlamydomonas reinhardtii, ukungafani kwe-physiological and cellular structure phakathi kweentlobo ze-microalgae ezahlukeneyo kuthetha ukuba i-microalgae nganye inokufuna ukwakhiwa kwe-cryoprotectant ethile. Xa kuthelekiswa nezisombululo ze-cryopreservation ezisetyenziswa kwezinye iindlela ze-microbial kunye nezilwanyana ze-cryopreservation, isisombululo se-cryopreservation se-microalgae kufuneka siqwalasele isakhiwo sodonga lweseli, ukuxhathisa iqabaka, kunye nokuphendula okunetyhefu okukhuselekileyo kwiiseli ze-algal zeentlobo ezahlukeneyo ze-algal.

Itekhnoloji ye-vitrification cryopreservation ye-microalgae isebenzisa izisombululo eziyilwe ngokukodwa ukugcina iiseli kumaqondo obushushu aphantsi kakhulu, njenge-nitrogen engamanzi okanye -80 ° C, emva kwenkqubo yokupholisa ecwangcisiweyo. Iikristale zomkhenkce zihlala zenza ngaphakathi kweeseli ngexesha lokupholisa, kubangela umonakalo kwisakhiwo seseli kunye nokulahleka komsebenzi weeseli, okukhokelela ekufeni kweeseli. Ukuze kuphuhliswe izisombululo ze-microalgae cryopreservation, i-protoga yenza uphando olunzulu kwiimpawu zeselula ze-microalgae, kuquka ukusabela kwazo kwizikhuseli ezahlukeneyo kunye nendlela yokunciphisa ngokufanelekileyo umonakalo obangelwa ngumkhenkce kunye noxinzelelo lwe-osmotic. Oku kubandakanya uhlengahlengiso oluqhubekayo kuhlobo, ugxininiso, ulandelelwano olongezelelweyo, ukupholisa kwangaphambili, kunye neenkqubo zokubuyisela ii-agent ezikhuselayo kwisisombululo se-cryopreservation, okubangelwa ukuphuhliswa kwesisombululo esibanzi se-microalgae cryopreservation esibizwa ngokuba yi-Froznthrive ™ kunye ne-teknoloji exhasayo ye-vitrification yokukhenkcela.