PRPTOGA MICROALGAE CDMO LITŠEBELETSO
- Laeborari ea Microalgae
Phepelo ea Peō ea Microalgae
▪ PROTOGA Microalgae Library e bolokile mefuta e ka bang lekholo ea maalgae, ho akarelletsa empa e sa felle feela ho Haematococcus Pluvialis, Chlorella sp., Dictyosphaerium sp., Scenedesmus sp. le Synechocystis sp.. Lipeo tsohle tsa algae li hloekisoa le ho netefatsoa e le li-microalgae tse khethehileng tse ka sebelisoang liphuputsong tsa saense.
Karohano ea Microalgae
▪ PROTOGA e ka arola le ho hloekisa li-microalgae tsa tlhaho matšeng, linōka, mekhoabo, tse ka hlahlojoang ka likhatello tse fapaneng (mocheso o phahameng / o tlaase, lefifi / leseli joalo-joalo). Bareki ba rona ba ka ba le li-microalgae tse hloekisitsoeng le tse hlahlobiloeng bakeng sa lipatlisiso, litokelo tsa molao, nts'etsopele ea khoebo.
Ho hlahisa liphatsa tsa lefutso
▪ PROTOGA e thehile mokhoa o sebetsang oa ARTP bakeng sa microalgae mutagenesis, haholo-holo e loketseng mefuta e meng e tloaelehileng. PROTOGA e ka boela ea haha sistimi e ncha ea ARTP le banka ea liphetoho ha ho hlokahala li-microalgae tse itseng.
- E tsitsitseng
Ha li bapisoa le oli ea tlhapi le lijo tsa liphoofolo, li-microalgae li tšoarella ebile li na le tikoloho. Microalgae e tla ba tharollo ea mathata a teng indastering ea lijo, temo le ho futhumala ha lefatše.
PROTOGA e ikemiselitse ho nts'etsapele mahlale a mahlale a manyane a potlakisang ntlafatso ea indasteri ea li-microalgae, ho thusa ho fokotsa koluoa ea lefats'e ea lijo, khaello ea matla le tšilafalo ea tikoloho. Re lumela hore microalgae e ka susumetsa lefatše le lecha leo batho ba phelang ka lona ka tsela e phetseng hantle le e tala.
- Tlhahiso e ikhethileng
Ho belisoa ha Microalgae & Post-Processing
i.PROTOGA e hahile ho feta 100 square metres ea C-level plant ho latela ISO Class7 le GMP, hammoho le kamore ea setso ea mocheso le mongobo le sebaka se hloekileng ho latela litlhoko tsa laesense ea tlhahiso ea lijo, e ka etsoang ho latela moreki. litlhoko.
ii.Re na le mefuta e fapaneng ea fermenters e ikemetseng ea 5L ho isa ho 1000L, e koahelang lab-scale to pilot-scale production.
iii.Post-processing e kenyelletsa pokello ea lisele, ho omisa, ho sila libolo joalo-joalo.
iv. Mehaho ea liteko le lisebelisoa tse kang HPLC le GC li etsa tlhahlobo ea lihlahisoa tsa biomass, carotenoids, fatty acids, organic carbon, nitrogen, phosphorus le lintho tse ling.
- Biology ea limolek'hule
Microalgal Plasmid Bank
▪ Microalgal Plasmid Bank e kenyelletsa empa e sa felle feela ho li-transformation plasmids tse tloaelehileng. Plasmid Bank e fana ka mefuta e fapaneng ea li-vector tse loketseng le tse sebetsang hantle bakeng sa lithuto tse fapaneng.
AI Optimization ea Gene Sequence
▪ PROTOGA e na le mokhoa oa ho ntlafatsa liphatsa tsa lefutso ka ho ithuta ka AI. Mohlala, e ka ntlafatsa ORF ka liphatsa tsa lefutso tse sa tloaelehang, ea hlokomela tatellano ea boemo bo holimo, ea thusa ho lebisa gene overexpress.
Ho feteletsa maikutlo ho Chlamydomonas reinhardtii
▪ PROTOGA's Chlamydomonas reinhardtii e entsoe ka chessis ea microalgal bakeng sa protheine e feteletseng e tšoailoeng ka HA, Strep kapa GFP. Ho ea ka litlhoko tsa hau, protheine e lebisitsoeng e ka hlahisoa ka cytoplasm kapa chloroplast.
Gene Knockout ho Chlamydomonas reinhardtii
▪ Sehlopha sa botekgeniki sa PROTOGA se hahile mokhoa oa ho lokisa oa Crispr/cas9 le Crispr/cas12a Chlamydomonas reinhardtii, ho kenyeletsoa moralo oa gRNA, donor DNA template, complex assembly le lintho tse ling, tse tsamaisang gene knockout le site-direct mutagenesis.