Polysaccharide e tsoang ho Chlorella (PFC), joalo ka polysaccharide ea tlhaho, e hohetse tlhokomelo e ngata ho tsoa ho litsebi lilemong tsa morao tjena ka lebaka la melemo ea eona ea chefo e tlase, litlamorao tse tlase, le litlamorao tse pharalletseng. Mesebetsi ea eona ea ho theola lipids ea mali, anti-tumor, anti-inflammatory, anti Parkinson's, anti-botsofali, joalo-joalo e tiisitsoe pele litekong tsa in vitro le in vivo. Leha ho le joalo, ho ntse ho na le lekhalo lipatlisisong tsa PFC joalo ka modulator ea 'mele oa motho.

Lisele tsa Dendritic (DCs) ke lisele tse matla ka ho fetisisa tse hlahisang li-antigen 'meleng oa motho. Palo ea li-DC 'meleng oa motho e nyane haholo, mme mohlala oa cytokine mediated in vitro in vitro, e leng peripheral blood mononuclear cell-derived DCs (moDCs), e sebelisoa hangata. Moetso oa in vitro o hlahisitsoeng ke DC o tlalehiloe ka lekhetlo la pele ka 1992, e leng mokhoa oa setso oa li-DC. Ka kakaretso, e hloka ho lengoa matsatsi a 6-7. Lisele tsa 'mele oa litoeba li ka hlahisoa ka granulocyte macrophage colony-stimulating factor (GM-CSF) le interleukin (IL) -4 ho fumana li-DC tse sa tloaelehang (sehlopha sa PBS). Li-cytokine li eketsoa e le li-stimuli tse hōlileng tsebong le tse hlahisitsoeng ka matsatsi a 1-2 ho fumana li-DC tse hōlileng tsebong. Phuputso e 'ngoe e tlalehile hore lisele tsa CD14 + tsa motho tse hloekisitsoeng li ne li entsoe ka interferon - β (IFN - β) kapa IL-4 bakeng sa matsatsi a 5, ebe li hlahisoa ka tumor necrosis factor-a (TNF-a) bakeng sa matsatsi a 2 ho fumana li-DC tse phahameng. polelo ea CD11c le CD83, tse nang le bokhoni bo matla ba ho khothalletsa ho ata ha lisele tsa CD4 + T tsa allogeneic le lisele tsa CD8 + T. Li-polysaccharides tse ngata tse tsoang mehloling ea tlhaho li na le ts'ebetso e ntle haholo ea ho itšireletsa mafung, joalo ka li-polysaccharides tse tsoang ho li-mushroom tsa shiitake, li-mushroom tsa gill tse arohaneng, li-mushroom tsa Yunzhi le Poria cocos, tse sebelisitsoeng ts'ebetsong ea bongaka. Li ka ntlafatsa ts'ebetso ea 'mele ea ho itšireletsa mafung, tsa matlafatsa sesole sa' mele, 'me tsa sebetsa e le litlhare tsa adjuvant bakeng sa kalafo ea anti-tumor. Leha ho le joalo, ho na le litlaleho tse fokolang tsa lipatlisiso mabapi le PFC e le modulator ea 'mele oa motho. Ka hona, sengoloa sena se etsa lipatlisiso tsa pele mabapi le karolo le mekhoa e amanang le eona ea PFC ho khothaletsa kholo ea li-moDC, molemong oa ho lekola bokhoni ba PFC joalo ka modulator ea tlhaho ea 'mele ea ho itšireletsa mafung.

Ka lebaka la karolo e tlase haholo ea li-DC liseleng tsa batho le paballo e phahameng ea mefuta lipakeng tsa litoeba tsa DC le li-DC tsa batho, molemong oa ho rarolla mathata a lipatlisiso a bakiloeng ke tlhahiso e tlase ea DC, mefuta ea in vitro induction ea li-DC tse nkiloeng liseleng tsa mali a mononuclear a motho. li ithutile, tse ka fumanang li-DC tse nang le immunogenicity e ntle ka nako e khuts'oane. Ka hona, thuto ena e sebelisitse mokhoa o tloaelehileng oa ho susumetsa batho ba DCs in vitro: co culturing rhGM CSF le rhIL-4 in vitro, ho fetola seaplane letsatsi le leng le le leng, le ho fumana li-DC tse sa pheleng ka letsatsi la 5th; Ka letsatsi la 6th, palo e lekanang ea PBS, PFC, le LPS e ile ea eketsoa ho ea ka lihlopha le tsa setso bakeng sa lihora tsa 24 e le protocol ea setso bakeng sa ho kenya li-DC tse tsoang liseleng tsa mali tsa peripheral mononuclear.

Li-polysaccharides tse tsoang lihlahisoa tsa tlhaho li na le melemo ea chefo e tlaase le theko e tlaase e le li-immunostimulants. Kamora liteko tsa pele, sehlopha sa rona sa lipatlisiso se fumane hore PFC e ntlafatsa haholo letšoao le butsoitseng la CD83 holim'a lisele tsa DC tse tsoang ka har'a lisele tsa motho tse tsoang ka har'a lisele tsa "mononuclear cell in vitro". Liphetho tsa cytometry tse phallang li bontšitse hore ho kenella ha PFC ka har'a mahloriso a 10 μ g / mL bakeng sa lihora tsa 24 ho ile ha fella ka pontšo ea tlhōrō ea letšoao le hōlileng tsebong la CD83 holim'a li-DC, tse bontšang hore li-DC li kene sebakeng se hōlileng tsebong. Ka hona, sehlopha sa rona sa lipatlisiso se ile sa khetha in vitro induction le leano la ho kenella. CD83 ke biomarker ea bohlokoa e butsoitseng ka holim'a li-DC, ha CD86 e sebetsa e le molek'hule ea bohlokoa ea co stimulatory holim'a li-DC, e sebetsa e le lets'oao la bobeli la ho kenya lisele tsa T. Polelo e ntlafalitsoeng ea li-biomarker tse peli tsa CD83 le CD86 e bonts'a hore PFC e khothaletsa kholo ea mali a motho a tsoang ka lisele tsa mononuclear, e fana ka maikutlo a hore PFC e ka eketsa ka nako e ts'oanang boemo ba secretion ea li-cytokine holim'a li-DC. Ka hona, thuto ena e ile ea hlahloba maemo a li-cytokines IL-6, TNF-a, le IL-10 tse patiloeng ke li-DC tse sebelisang ELISA. IL-10 e amana haufi-ufi le mamello ea 'mele ea ho itšireletsa mafung ea DC,' me li-DC tse nang le mamello ea 'mele ea ho itšireletsa mafung li atisa ho sebelisoa kalafo ea hlahala, ho fana ka maikutlo a ka 'nang a phekoloa bakeng sa ho mamellana ha' mele oa ho itšireletsa mafung ka ho fetisetsa litho tsa 'mele; Lelapa la 1L-6 le phetha karolo ea bohlokoa ho itšireletsa ka tlhaho le ho ikamahanya le maemo, hematopoiesis, le liphello tse khahlanong le ho ruruha; Ho na le lithuto tse bontšang hore IL-6 le TGF β li kopane hammoho ho arola lisele tsa Th17; Ha 'mele o hlaseloa ke kokoana-hloko, TNF-a e hlahisoang ke DCs ho arabela ts'ebetsong ea kokoana-hloko e sebetsa e le autocrine maturation factor ho khothalletsa DC maturation. Ho thibela TNF-a ho tla beha li-DC boemong bo sa tsitsang, ho ba thibela ho sebelisa ka botlalo mosebetsi oa bona oa tlhahiso ea antigen. Lintlha tsa ELISA thutong ena li bontšitse hore boemo ba sephiri ba IL-10 sehlopheng sa PFC bo eketsehile haholo ha bo bapisoa le lihlopha tse ling tse peli, e leng se bontšang hore PFC e ntlafatsa ho mamellana ha 'mele oa DCs; Maemo a ntseng a eketseha a secretion a IL-6 le TNF-a a fana ka maikutlo a hore PFC e kanna ea ba le phello ea ho ntlafatsa DC ho khothaletsa phapang ea lisele tsa T.