Likarolong tse fapaneng tsa lipatlisiso le ts'ebeliso ea li-microalgae, theknoloji ea ho boloka nako e telele ea lisele tsa microalgae e bohlokoa. Mekhoa e tloaelehileng ea ho boloka maalgae a tobana le mathata a mangata, ho kenyelletsa le ho fokotseha ha botsitso ba liphatsa tsa lefutso, litšenyehelo tse eketsehileng, le likotsi tse eketsehileng tsa tšilafalo. Ho sebetsana le litaba tsena, protoga e thehile mokhoa oa vitrification cryopreservation o loketseng maalga a fapaneng. Ho etsoa ha tharollo ea cryopreservation ho bohlokoa bakeng sa ho boloka matla le botsitso ba liphatsa tsa lefutso tsa lisele tsa microalgae.

Hona joale, le hoja likopo tse atlehileng li entsoe ho Chlamydomonas reinhardtii, phapang ea sebopeho sa 'mele le lisele pakeng tsa mefuta e sa tšoaneng ea microalgae e bolela hore microalgae e' ngoe le e 'ngoe e ka hloka mekhoa e khethehileng ea cryoprotectant. Ha ho bapisoa le litharollo tsa cryopreservation tse sebelisoang mekhoeng e meng ea cryopreservation ea likokoana-hloko le liphoofolo, tharollo ea cryopreservation bakeng sa microalgae e hloka ho nahana ka sebopeho sa lerako la lisele, ho hanyetsa serame, le karabelo e khethehileng ea chefo ea li sireletsang liseleng tsa microalgae tsa mefuta e fapaneng ea algal.

Theknoloji ea vitrification cryopreservation ea microalgae e sebelisa litharollo tsa cryopreservation tse etselitsoeng ka ho khetheha ho boloka lisele ka mocheso o tlase haholo, joalo ka metsi a naetrojene kapa -80 ° C, kamora ts'ebetso e hlophisitsoeng ea ho pholisa. Hangata likristale tsa leqhoa li theha ka har'a lisele nakong ea pholileng, tse bakang tšenyo ea sebopeho sa lisele le tahlehelo ea ts'ebetso ea lisele, e leng se lebisang lefung la sele. E le ho hlahisa litharollo tsa microalgae cryopreservation, protoga e ile ea etsa lipatlisiso tse tebileng ka litšobotsi tsa lisele tsa microalgae, ho kenyelletsa le karabelo ea tsona ho li sireletsang tse fapaneng le mokhoa oa ho fokotsa tšenyo e bakoang ke leqhoa le khatello ea osmotic. Sena se kenyelletsa liphetoho tse tsoelang pele ho mofuta, mahloriso, tatellano ea ho eketsa, ho pholisa pele, le mekhoa ea ho hlaphoheloa ea li-agent tse sireletsang ka har'a tharollo ea cryopreservation, e leng se hlahisang nts'etsopele ea tharollo ea microalgae cryopreservation e pharaletseng e bitsoang Froznthrive ™ Le theknoloji e tšehetsang vitrification ea leqhoa.