Polysaccharide ka Chlorella (PFC), sida polysaccharide dabiiciga ah, ayaa soo jiidatay dareen badan oo ka yimid culimada sannadihii la soo dhaafay sababtoo ah faa'iidooyinka sunta hoose, waxyeellooyin hoose, iyo saameyn ballaaran. Shaqadeeda hoos u dhigista xuubka dhiigga, buro-ka-hortagga, ka-hortagga-bararka, ka-hortagga Parkinson, ka-hortagga gabowga, iwm. ayaa si hordhac ah loo ansixiyay in vitro iyo tijaabooyinka vivo. Si kastaba ha ahaatee, weli waxaa jira farqi u dhexeeya cilmi-baarista PFC sida modulator-ka difaaca aadanaha.

Unugyada Dendritic (DCs) waa unugyada soo-bandhigidda antigen-ka ee ugu awoodda badan ee jidhka bini'aadamka. Tirada DC-yada ku jira jidhka bini'aadamka aad bay u yar yihiin, iyo cytokine dhexdhexaadin ah qaabka induction in vitro, oo ah unugyada dhiigga ee mononuclear cell-derived DCs (moDCs), ayaa caadi ahaan loo isticmaalaa. Qaabka in vitro induced DC ayaa markii ugu horeysay la soo sheegay 1992, kaas oo ah nidaamka dhaqanka ee DC-yada. Guud ahaan, waxay u baahan tahay beerashada 6-7 maalmood. Unugyada dhuuxa jiirka waxaa lagu dhaqi karaa granulocyte macrophage colony-stimulating factor (GM-CSF) iyo interleukin (IL) -4 si loo helo DCs aan qaan-gaarin (kooxda PBS). Cytokines waxaa lagu daraa sida kicinta qaan-gaarka ah waxaana la dhaqangelinayaa 1-2 maalmood si loo helo DC-yada qaan-gaarka ah. Daraasad kale ayaa sheegtay in unugyada CD14 + ee bini'aadamka ee la nadiifiyey lagu dhaqay interferon - β (IFN - β) ama IL-4 maalmaha 5, ka dibna lagu beeray buro necrosis factor-a (TNF-a) maalmo 2 si loo helo DC-yada sare. muujinta CD11c iyo CD83, kuwaas oo awood xoog leh u leh inay kor u qaadaan faafinta unugyada CD4 + T ee allogeneic iyo unugyada CD8 + T. Noocyo badan oo polysaccharides ah oo laga helay ilo dabiici ah ayaa leh firfircooni is-difaaceed oo heer sare ah, sida polysaccharides ee boqoshaada shiitake, boqoshaada gill ee kala qaybsan, boqoshaada Yunzhi, iyo Poria cocos, kuwaas oo lagu dabaqay waxqabadka kiliinikada. Waxay si wax ku ool ah u wanaajin karaan shaqada difaaca jirka, waxay kor u qaadi karaan difaaca, waxayna u adeegi karaan daawayn adjuvant ee daawaynta ka hortagga burada. Si kastaba ha ahaatee, waxaa jira warbixino yar oo cilmi baaris ah oo ku saabsan PFC sida modulator-ka difaaca aadanaha. Sidaa darteed, maqaalkani waxa uu sameeyaa cilmi-baadhis horudhac ah oo ku saabsan doorka iyo hababka la xidhiidha PFC si kor loogu qaado korriinka moDCs, si loo qiimeeyo kartida PFC sida modulator-ka difaaca dabiiciga ah.

Sababtoo ah saamiga aadka u hooseeya ee DC-yada unugyada bini'aadamka iyo ilaalinta noocyada sare ee noocyada kala duwan ee u dhexeeya jiirka DC-yada iyo DC-yada bini'aadamka, si loo xalliyo dhibaatooyinka cilmi-baarista ee ay sababtay wax soo saarka DC ee hooseeya, moodooyinka induction in vitro ee DC-yada laga soo qaatay unugyada mononuclear ee dhiigga bini'aadamka. la dersay, kuwaas oo heli kara DC-yo leh difaac wanaagsan muddo gaaban gudaheed. Sidaa darteed, daraasaddan waxay adeegsatay habka dhaqameed ee kicinta DC-yada bini'aadamka ee vitro: co culturing rhGM CSF iyo rhIL-4 in vitro, beddelidda dhexdhexaadka maalin kasta oo kale, iyo helitaanka DCs aan qaan-gaarin maalinta 5aad; Maalintii 6aad, tiro le'eg oo PBS, PFC, iyo LPS ah ayaa lagu daray iyadoo loo eegayo kooxaynta iyo dhaqanka 24 saacadood iyadoo hab-maamuuska dhaqanka ee kicinta DC-yada laga soo qaatay unugyada mononuclear-ka ee bini'aadamka.

Polysaccharides oo ka soo jeeda alaabada dabiiciga ah waxay leeyihiin faa'iidooyinka sunta hoose iyo qiimaha jaban sida immunostimulants. Tijaabooyin horudhac ah ka dib, kooxdayada cilmi-baarista waxay ogaadeen in PFC ay si weyn u wanaajiso sumadda CD83 ee dusha sare ee unugyada unugyada DC-ka ee unugyadu ka soo jeedo ee vitro. Natiijooyinka cytometry socodka qulqulka ayaa muujiyay in faragelinta PFC ee uruurinta 10 μg/mL ee 24 saacadood ay keentay muujinta ugu sarreysa ee calaamadeeyaha qaan gaadhka ah ee CD83 ee dusha DC-yada, taas oo muujinaysa in DCs ay galeen xaalad qaan-gaar ah. Sidaa darteed, kooxdayada cilmi-baadhistu waxay go'aamiyeen qorshaha dhexgalka iyo galitaanka in vitro. CD83 waa biomarker muhim ah oo baaluq ah oo ku yaal dusha DC-yada, halka CD86 uu u adeego sidii unug firfircoon oo muhiim ah oo ku yaal dusha DC-yada, isaga oo u dhaqmaya sidii calaamadda labaad ee firfircoonida unugyada T. Muujinta la xoojiyey ee labada biomarkers CD83 iyo CD86 waxay muujineysaa in PFC ay kor u qaaddo korriinka dhiigga bini'aadamka ee unugyada mononuclear-ka ee DC-yada, taasoo soo jeedinaysa in PFC ay isku mar kordhin karto heerka dheecaanka cytokines ee dusha DC-yada. Sidaa darteed, daraasaddan ayaa qiimeysay heerarka cytokines IL-6, TNF-a, iyo IL-10 ee ay siiyeen DC-yada iyadoo la adeegsanayo ELISA. IL-10 waxay si dhow ula xiriirtaa dulqaadka difaaca ee DC-yada, iyo DC-yada leh dulqaadka difaaca ayaa caadi ahaan loo isticmaalaa daaweynta burooyinka, siinta fikradaha daweynta ee suurtogalka ah ee dulqaadka difaaca ee ku-tallaalidda xubnaha; Qoyska 1L-6 waxay door muhiim ah ka ciyaaraan difaaca gudaha iyo la qabsiga, hematopoiesis, iyo saamaynta anti-bararka; Waxaa jira daraasado muujinaya in IL-6 iyo TGF β si wadajir ah uga qaybqaataan kala-soocidda unugyada Th17; Marka jirku soo galo fayras, TNF-a oo ay soo saartay DCs iyada oo ka jawaabaysa firfircoonida fayraska waxay u shaqeysaa sidii cunsur korriin autocrine si kor loogu qaado korriinka DC. Joojinta TNF-a waxay DC-yada gelin doontaa marxalad aan qaan-gaarin, taasoo ka ilaalinaysa inay si buuxda u fuliso shaqadooda soo bandhigida antigen-ka. Xogta ELISA ee daraasaddan ayaa muujisay in heerka qarsoodiga ah ee IL-10 ee kooxda PFC uu si weyn u kordhay marka la barbardhigo labada kooxood ee kale, taas oo muujinaysa in PFC ay kor u qaaddo dulqaadka difaaca ee DC-yada; Heerarka qarsoodiga ah ee sii kordhaya ee IL-6 iyo TNF-a waxay soo jeedinayaan in PFC laga yaabo inay saameyn ku yeelato kor u qaadida DC si kor loogu qaado kala duwanaanshaha unugyada T.