Qaybaha kala duwan ee cilmi-baarista microalgae iyo codsiga, tignoolajiyada ilaalinta muddada dheer ee unugyada microalgae waa muhiim. Hababka ilaalinta microalgae-dhaqameedka ayaa wajahaya caqabado badan, oo ay ku jiraan xasilloonida hidde-sidaha oo yaraatay, kharashyada oo kordhay, iyo khatarta wasakhowga oo kordhay. Si wax looga qabto arrimahan, protoga ayaa soo saartay farsamada faylalka ilaalinta ee ku habboon microalgae kala duwan. Samaynta xalka cryopreservation ayaa muhiim u ah ilaalinta firfircoonida iyo xasilloonida hidde ee unugyada microalgae.

Waqtigan xaadirka ah, in kasta oo codsiyo guul leh lagu sameeyay Chlamydomonas reinhardtii, kala duwanaanshaha qaabdhismeedka jireed iyo unugga ee u dhexeeya noocyada kala duwan ee microalgae macnaheedu waa in microalgae kasta uu u baahan karo qaabab gaar ah oo loo yaqaan 'cryptoprotectant formulas. Marka la barbardhigo xalalka cryopreservation ee loo isticmaalo farsamooyinka kale ee unugyada microbial iyo xayawaanka, xalka cryopreservation ee microalgae wuxuu u baahan yahay inuu tixgeliyo qaab dhismeedka derbiga unugyada, caabbinta dhaxan, iyo falcelinta sunta gaarka ah ee ilaalinta unugyada microalgae ee noocyada kala duwan ee algae.

Tiknoolajiyada kaydinta vitrification ee microalgae waxay isticmaashaa xalal gaar ah oo loogu talagalay cryopreservation si ay ugu kaydiso unugyada heerkulka aadka u hooseeya, sida nitrogen dareere ah ama -80 ° C, ka dib habka qaboojinta barnaamijka. Karistaanka barafka sida caadiga ah waxay ku sameeyaan gudaha unugyada inta lagu jiro qaboojinta, taasoo keenta dhaawac soo gaara qaab dhismeedka unugyada iyo luminta shaqada unugga, taasoo keenta dhimashada unugyada. Si loo horumariyo xalalka cryopreservation microalgae, protoga waxay samaysay cilmi baaris qoto dheer oo ku saabsan sifooyinka gacanta ee microalgae, oo ay ku jiraan falcelintooda ilaalo kala duwan iyo sida ugu waxtarka leh ee loo yareeyo waxyeelada ay keento qabowga iyo cadaadiska osmotic. Tan waxaa ka mid ah hagaajinta joogtada ah ee nooca, fiirsashada, isku dhafka dheeriga ah, qaboojinta hore, iyo hababka soo kabashada ee wakiilada ilaalinta ee xalka cryopreservation, taasoo keentay horumarinta xalal ballaadhan oo microalgae cryopreservation xal loo yaqaan Froznthrive ™ iyo taageerada tignoolajiyada qaboojinta vitrification.