Polysaccharide kubva kuChlorella (PFC), seyakasikwa polysaccharide, yakakwezva kutarisisa kwakawanda kubva kuvadzidzi mumakore achangopfuura nekuda kwezvakanaka zvayo zvehupfu hwakadzika, mhedzisiro yakaderera, uye yakakura-spectrum mhedzisiro. Mabasa ayo mukudzikisa ropa lipids, anti-bundu, anti-kuzvimba, anti Parkinson's, anti-kuchembera, nezvimwe zvakambosimbiswa mu vitro uye mu vivo kuyedza. Nekudaro, pachine gaka mukutsvagisa kwePFC senge munhu immune immune modulator.

Maseru eDendritic (DCs) ndiwo ane simba zvakanyanya akasarudzika antigen-anopa maseru mumuviri wemunhu. Huwandu hweDCs mumuviri wemunhu idiki zvakanyanya, uye cytokine mediated in vitro induction modhi, iyo yemunhu peripheral blood mononuclear cell-derived DCs (moDCs), inowanzoshandiswa. Iyo in vitro induced DC modhi yakatanga kutaurwa muna 1992, inova yetsika tsika system yeDCs. Kazhinji, inoda kurima kwemazuva 6-7. Mouse bone mwongo masero anogona kugadzirwa ne granulocyte macrophage colony-stimulating factor (GM-CSF) uye interleukin (IL) -4 kuwana immature DCs (PBS boka). Cytokines inowedzerwa seyakakura kukurudzira uye yakasimwa kwemazuva 1-2 kuti iwane yakakura DCs. Imwe ongororo yakashuma kuti masero eCD14 + akacheneswa akagadzirwa ne interferon - β (IFN - β) kana IL-4 kwemazuva 5, uye akazogadzirwa ne tumor necrosis factor-a (TNF-a) kwemazuva maviri kuti awane DCs nepamusoro. kutaura kweCD11c uye CD83, iyo ine simba rakasimba rekusimudzira kupararira kweallogeneic CD4 + T masero uye CD8 + T masero. Mapolysaccharides akawanda kubva kune zvakasikwa ane yakanakisa immunomodulatory chiitiko, senge polysaccharides kubva kune shiitake howa, split gill howa, Yunzhi howa, uye Poria cocos, ayo akaiswa mukiriniki maitiro. Vanogona kunyatso vandudza immune system yemuviri, kuwedzera kusadzivirirwa kwemuviri, uye kushanda seadjuvant kurapa kweanti-tumor kurapwa. Nekudaro, kune mashoma ekutsvagisa mishumo paPFC seyemunhu immune modulator. Naizvozvo, chinyorwa ichi chinoitisa tsvakiridzo yekutanga pamusoro pebasa uye nzira dzakabatana dzePFC mukusimudzira kukura kwemoDCs, kuitira kuongorora kugona kwePFC seyakasikwa immune modulator.

Nekuda kwechikamu chakanyanya kuderera cheDCs munyama dzevanhu uye nepamusoro-soro kuchengetedza pakati pemakonzo DCs nevanhu DCs, kuitira kugadzirisa matambudziko ekutsvagisa anokonzerwa nekushomeka kweDC kugadzirwa, in vitro induction modhi yeDCs inotorwa kubva muropa remunhu mononuclear maseru. dzakadzidzwa, dzinogona kuwana maDC ane immunogenicity yakanaka munguva pfupi. Nokudaro, chidzidzo ichi chakashandisa nzira yekare yekunyengedza vanhu DCs in vitro: co culturing rhGM CSF uye rhIL-4 in vitro, kushandura svikiro zuva rimwe nerimwe, uye kuwana maDC asina kusimba pazuva re5; Pazuva re6th, mavhoriyamu akaenzana ePBS, PFC, uye LPS akawedzerwa maererano nemapoka uye akagadzirwa kwemaawa makumi maviri nemana setsika yeprotocol yekupinza DCs inotorwa kubva kumunhu peripheral blood mononuclear cells.

Polysaccharides inotorwa kubva muzvigadzirwa zvechisikigo ine zvakanakira kuderera kwehupfu uye mutengo wakaderera se immunostimulants. Mushure mekuedza kwekutanga, boka redu rekutsvagisa rakaona kuti PFC inosimudzira zvakanyanya mamaki CD83 pamusoro peropa remunhu mononuclear cell-inotorwa DC maseru akaiswa mu vitro. Kuyerera kwe cytometry mhedzisiro yakaratidza kuti kupindira kwePFC pachikamu che10 μ g / mL kwemaawa makumi maviri nemana kwakaguma nekutaura kwepamusoro kweiyo mature marker CD83 pamusoro peDCs, zvichiratidza kuti DCs yakapinda munyika yakakura. Naizvozvo, boka redu rekutsvagisa rakasarudza iyo in vitro induction uye chirongwa chekupindira. CD83 yakakosha yakakura biomarker pamusoro peDCs, nepo CD86 inoshanda seyakakosha co stimulatory molecule pamusoro peDCs, ichiita sechiratidzo chechipiri chekumisikidza T masero. Kuwedzeredzwa kwekutaura kwema biomarkers maviri CD83 uye CD86 inoratidza kuti PFC inosimudzira kugadzirwa kweropa remunhu mononuclear cell-derived DCs, zvichiratidza kuti PFC inogona panguva imwe chete kuwedzera secretion level ye cytokines pamusoro peDCs. Nokudaro, chidzidzo ichi chakaongorora mazinga e-cytokines IL-6, TNF-a, uye IL-10 yakavanzwa neDCs vachishandisa ELISA. IL-10 yakanyatsobatana nekudzivirira kwekudzivirira kweDDCs, uye maDC ane simba rekudzivirira muviri anowanzoshandiswa mukurapa bundu, achipa mazano ekurapa maitiro ekushivirira kwemuviri mukugadzirisa nhengo; Mhuri ye1L-6 inoita basa rinokosha mukudzivirira kwekuzvarwa uye kugadzirisa, hematopoiesis, uye anti-inflammatory effects; Pane zvidzidzo zvinoratidza kuti IL-6 uye TGF β pamwe chete inobatanidzwa mukusiyanisa kweTh17 masero; Kana muviri uchinge wapinda nehutachiona, TNF-a inogadzirwa neDCs mukupindura hutachiona activation inoita se autocrine maturation factor kukurudzira DC maturation. Kuvhara TNF-a kuchaisa maDC muchikamu chisati chaitika, ichivadzivirira kubva pakuita zvizere basa ravo rekuratidzira antigen. Nhoroondo yeELISA muchidzidzo ichi yakaratidza kuti kuvharidzirwa kwe IL-10 muboka rePFC kwakawedzera zvakanyanya kuenzaniswa nemamwe mapoka maviri, zvichiratidza kuti PFC inowedzera kushivirira kweDDCs; Kuwedzera kwekuvanzika kwemazinga eIL-6 uye TNF-a anoratidza kuti PFC inogona kuve nemhedzisiro yekusimudzira DC kukurudzira T cell kusiyanisa.