Munzvimbo dzakasiyana-siyana dzekutsvagisa nekushandisa microalgae, tekinoroji yekuchengetedza kwenguva refu kwemasero emicroalgae yakakosha. Nzira dzechinyakare dzekuchengetedza microalgae dzinotarisana nematambudziko akawanda, kusanganisira kudzikira kwemajini, kuwedzera kwemitengo, uye kuwedzera njodzi dzekusvibiswa. Kugadzirisa nyaya idzi, protoga yakagadzira vitrification cryopreservation tekinoroji yakakodzera kune akasiyana ma microalgae. Kugadzirwa kweiyo cryopreservation solution kwakakosha pakuchengetedza hutano uye kugadzikana kwemajini emasero emicroalgae.

Parizvino, kunyangwe zvikumbiro zvakabudirira zvakaitwa paChlamydomonas reinhardtii, iyo physiological uye cellular structural misiyano pakati pemhando dzakasiyana dzemicroalgae zvinoreva kuti microalgae yega yega inogona kuda chaiyo cryoprotectant formulations. Kuenzaniswa nemhinduro dze cryopreservation dzinoshandiswa mune mamwe ma microbial uye mhuka cell cryopreservation maitiro, iyo cryopreservation solution ye microalgae inoda kufunga nezvesero madziro chimiro, chando kuramba, uye chaiyo muchetura kuita kweanodzivirira kune microalgae masero emarudzi akasiyana algal.

Iyo vitrification cryopreservation tekinoroji ye microalgae inoshandisa yakanyatso gadzirwa cryopreservation mhinduro kuchengetedza maseru pane yakanyanya kudzika tembiricha, senge mvura nitrogen kana -80 ° C, mushure mehurongwa hwekutonhora. Makristasi echando anowanzo kuumba mukati memasero panguva yekutonhora, zvichikonzera kukuvadzwa kwesero chimiro uye kurasikirwa kwesero kushanda, zvichitungamira kufa kwesero. Kugadzira microalgae cryopreservation mhinduro, protoga yakaita tsvakiridzo yakadzama pamusoro pemaserura maitiro eiyo microalgae, kusanganisira maitiro avo kune akasiyana anodzivirira uye maitiro ekudzikisa zvakanyanya kukuvara kunokonzerwa nechando uye osmotic pressure. Izvi zvinosanganisira kuenderera kugadziridzwa kurudzi, kutarisisa, kutevedzana kwekuwedzera, kutonhora kwekutanga, uye kudzoreredza maitiro ekudzivirira mune cryopreservation mhinduro, zvichikonzera kuvandudzwa kweyakakura-spectrum microalgae cryopreservation solution inonzi Froznthrive ™ Uye inotsigira vitrification yechando tekinoroji.