Polysaccharide yo muri Chlorella (PFC), nka polysaccharide isanzwe, yashishikaje cyane intiti mu myaka yashize kubera ibyiza by’uburozi buke, ingaruka nke, n'ingaruka zagutse. Ibikorwa byayo mukugabanya lipide yamaraso, kurwanya ibibyimba, kurwanya inflammatory, kurwanya Parkinson, kurwanya gusaza, nibindi byemejwe mbere na mbere muri vitro no mubushakashatsi bwa vivo. Nyamara, haracyari icyuho mubushakashatsi kuri PFC nkumuntu uhindura umubiri.

Ingirabuzimafatizo ya Dendritic (DCs) nizo ngirabuzimafatizo zikomeye zerekana umubiri wa muntu. Umubare wa DC mu mubiri wumuntu ni muto cyane, kandi cytokine yunganirwa muburyo bwa vitro induction, aribwo abantu bakoreshwa na peripheri yamaraso mononuclear selile ikomoka kuri DC (moDCs). Muri vitro iterwa na moderi ya DC yavuzwe bwa mbere mu 1992, aribwo buryo bwa gakondo bwumuco wa DC. Mubisanzwe, bisaba guhinga iminsi 6-7. Utugingo ngengabuzima two mu magufa dushobora guterwa hamwe na granulocyte macrophage colony-itera imbaraga (GM-CSF) na interleukin (IL) -4 kugirango ubone DC zidakuze (itsinda rya PBS). Cytokine yongewemo nkibikuze kandi ikuze muminsi 1-2 kugirango ibone DC ikuze. Ubundi bushakashatsi bwerekanye ko selile CD14 + yumuntu yatunganijwe yakoreshwaga na interferon - β (IFN - β) cyangwa IL-4 muminsi 5, hanyuma igahingwa hamwe nikibyimba cya nekrosis-a (TNF-a) muminsi 2 kugirango ibone DC zifite hejuru imvugo ya CD11c na CD83, zifite imbaraga zikomeye zo guteza imbere ikwirakwizwa rya selile allogeneic CD4 + T na selile CD8 + T. Polysaccharide nyinshi ziva mumasoko karemano zifite ibikorwa byiza byo gukingira indwara, nka polysaccharide iva ibihumyo bya shiitake, ibihumyo bya gill, ibihumyo bya Yunzhi, na coco ya Poria, byakoreshejwe mubikorwa byubuvuzi. Zishobora kunoza imikorere yumubiri yumubiri, kongera ubudahangarwa, no kuba imiti ivura imiti igabanya ubukana. Ariko, hari raporo zubushakashatsi kuri PFC nkumuntu uhindura umubiri. Kubwibyo, iyi ngingo ikora ubushakashatsi bwibanze ku ruhare n’uburyo bujyanye na PFC mu guteza imbere imikurire ya moDCs, hagamijwe gusuzuma ubushobozi bwa PFC nkumuntu uhindura umubiri.

Bitewe numubare muke cyane wa DC mubice byumuntu hamwe no kubungabunga amoko menshi hagati yimbeba DC na DC zabantu, kugirango bikemure ibibazo byubushakashatsi byatewe numusaruro muke wa DC, muburyo bwa vitro induction ya DC ikomoka kumasemburo ya monone nucléaire ya muntu; byizwe, bishobora kubona DC hamwe nubudahangarwa bwiza mugihe gito. Kubwibyo, ubu bushakashatsi bwakoresheje uburyo gakondo bwo gukurura DC zabantu muri vitro: co guhuza rhGM CSF na rhIL-4 muri vitro, guhindura uburyo buri munsi, no kubona DC idakuze kumunsi wa 5; Ku munsi wa 6, ingano ingana ya PBS, PFC, na LPS yongewemo ukurikije amatsinda kandi ikagira umuco mugihe cyamasaha 24 nka protocole yumuco yo gutera DC ikomoka kumasemburo yamaraso ya monone nucléaire.

Polysaccharide ikomoka ku bicuruzwa karemano bifite ibyiza byuburozi buke nigiciro gito nka immunostimulants. Nyuma yubushakashatsi bwibanze, itsinda ryacu ryubushakashatsi ryasanze PFC yongerera cyane ikimenyetso cya CD83 gikuze hejuru yumuntu wa peripheri yamaraso ya mononuclear selile ikomoka muri DC yatewe muri vitro. Ibisubizo bya cytometrie byerekanaga ko kwivanga kwa PFC kumurongo wa 10 μ g / mL kumasaha 24 byatumye habaho imvugo yerekana ibimenyetso bikuze CD83 ikuze hejuru ya DC, byerekana ko DC yinjiye muburyo bukuze. Kubwibyo, itsinda ryacu ryubushakashatsi ryagennye muri vitro induction na gahunda yo gutabara. CD83 ni biomarker yingenzi ikuze hejuru ya DC, mugihe CD86 ikora nka molekile yingenzi itera imbaraga hejuru ya DC, ikora nkikimenyetso cya kabiri cyo gukora selile T. Imvugo ishimishije yerekana biomarkers ebyiri CD83 na CD86 yerekana ko PFC iteza imbere gukura kwamaraso ya peripheri yamaraso ya mononuclear selile ikomoka kuri DC, byerekana ko PFC ishobora icyarimwe kongera urwego rwimyunyungugu ya cytokine hejuru ya DC. Kubwibyo, ubu bushakashatsi bwasuzumye urwego rwa cytokine IL-6, TNF-a, na IL-10 rwasohowe na DC ukoresheje ELISA. IL-10 ifitanye isano rya bugufi no kwihanganira ubudahangarwa bw'umubiri wa DC, kandi DC ifite kwihanganira ubudahangarwa bw'umubiri ikoreshwa cyane mu kuvura ibibyimba, itanga ibitekerezo byo kuvura bishobora kwihanganira ubudahangarwa bw'umubiri mu guhinduranya ingingo; Umuryango wa 1L-6 ugira uruhare runini mubudahangarwa bw'umubiri no guhuza n'imihindagurikire y'ikirere, hematopoiesis, n'ingaruka zo kurwanya inflammatory; Hariho ubushakashatsi bwerekana ko IL-6 na TGF β bafatanya mugutandukanya selile Th17; Iyo umubiri wibasiwe na virusi, TNF-yakozwe na DCs kugirango isubize ibikorwa bya virusi ikora nkikintu cyo gukura kwa autocrine kugirango iteze imbere gukura kwa DC. Guhagarika TNF-a bizashyira DC murwego rutarakura, bibabuze gukora byimazeyo ibikorwa byabo byo kwerekana antigen. Amakuru ya ELISA muri ubu bushakashatsi yerekanye ko urwego rw’ibanga rwa IL-10 mu itsinda rya PFC rwiyongereye ku buryo bugaragara ugereranije n’andi matsinda yombi, byerekana ko PFC yongerera ubudahangarwa bw'umubiri DC; Urwego rwiyongera rwurwego rwa IL-6 na TNF-byerekana ko PFC ishobora kugira ingaruka zo kuzamura DC kugirango iteze imbere T selile.