Mubice bitandukanye byubushakashatsi bwa microalgae no kubishyira mubikorwa, tekinoroji yo kubungabunga igihe kirekire ingirabuzimafatizo ya microalgae ni ngombwa. Uburyo bwa gakondo bwo kubungabunga microalgae burahura ningorane nyinshi, zirimo kugabanuka kwimiterere yimiterere yabantu, kongera ibiciro, hamwe n’ingaruka ziterwa n’umwanda. Kugira ngo ibyo bibazo bikemuke, protoga yashyizeho uburyo bwa vitrification cryopreservation tekinike ikwiranye na microalgae zitandukanye. Gutegura igisubizo cya cryopreservation ningirakamaro mugukomeza ubuzima ningirabuzimafatizo ya selile ya microalgae.

Kugeza ubu, nubwo byakozwe neza kuri Chlamydomonas reinhardtii, itandukaniro ryimiterere ya physiologique na selile hagati yubwoko butandukanye bwa microalgae bivuze ko buri microalgae ishobora gusaba imiti yihariye. Ugereranije n’ibisubizo bya cryopreservation bikoreshwa mu bundi buryo bwa mikorobe n’inyamaswa zo mu bwoko bwa cryopreservation, igisubizo cya cryopreservation kuri microalgae gikeneye gutekereza ku miterere y’urukuta rw’akagari, kurwanya ubukonje, hamwe n’uburozi bw’uburozi bw’abashinzwe kurinda selile ya microalgae y’ubwoko butandukanye bwa algal.

Vitrification cryopreservation tekinoroji ya microalgae ikoresha ibisubizo byabugenewe byabitswe kugirango bibike selile mubushyuhe buke cyane, nka azote yuzuye cyangwa -80 ° C, nyuma yuburyo bukonje bwateguwe. Ububiko bwa kirisiti isanzwe ikora imbere muri selile mugihe cyo gukonja, bigatera kwangirika kwimikorere no gutakaza imikorere yimikorere, biganisha ku rupfu. Mu rwego rwo guteza imbere ibisubizo bya microalgae cryopreservation, protoga yakoze ubushakashatsi bwimbitse ku miterere ya selile ya microalgae, harimo n’imyitwarire yabo ku barinzi batandukanye ndetse n’uburyo bwo kugabanya cyane ibyangijwe n’igitutu n’umuvuduko wa osmotic. Ibi bikubiyemo guhora uhinduranya ubwoko, kwibanda, gukurikiranya, gukonjesha mbere, hamwe nuburyo bwo gukiza ibintu birinda igisubizo cya cryopreservation, bikavamo iterambere ryagutse rya microalgae cryopreservation solution yitwa Froznthrive ™ Nubuhanga bwa vitrification bukonjesha.