Polysaccharide yochokera ku Chlorella (PFC), ngati polysaccharide yachilengedwe, yakopa chidwi kwambiri ndi akatswiri m'zaka zaposachedwa chifukwa cha zabwino zake zapoizoni yochepa, zotsatira zake zochepa, komanso zotsatira zake. Ntchito zake pochepetsa lipids m'magazi, anti-chotupa, anti-inflammatory, anti Parkinson's, anti-kukalamba, ndi zina zambiri zatsimikiziridwa kale mu vitro ndi mu vivo. Komabe, pakadali kusiyana pakufufuza kwa PFC ngati modulitsa chitetezo chamunthu.

Ma cell a dendritic (DCs) ndi maselo amphamvu kwambiri owonetsa antigen m'thupi la munthu. Chiwerengero cha ma DC m'thupi la munthu ndi chochepa kwambiri, ndipo cytokine mediated in vitro induction model, yomwe ndi magazi aumunthu amtundu wa mononuclear cell-derived DCs (moDCs), amagwiritsidwa ntchito kwambiri. Mtundu wa in vitro wopangidwa ndi DC udanenedwa koyamba mu 1992, yomwe ndi chikhalidwe chachikhalidwe cha ma DC. Nthawi zambiri, zimafunikira kulima kwa masiku 6-7. Maselo a mafupa a mbewa amatha kupangidwa ndi granulocyte macrophage colony-stimulating factor (GM-CSF) ndi interleukin (IL) -4 kuti apeze ma DCs (gulu la PBS). Ma cytokines amawonjezedwa ngati zolimbikitsa zokhwima ndikukula kwa masiku 1-2 kuti apeze ma DC okhwima. Kafukufuku wina adanenanso kuti maselo oyeretsedwa a CD14 + aumunthu adapangidwa ndi interferon - β (IFN - β) kapena IL-4 kwa masiku a 5, kenako amakula ndi tumor necrosis factor-a (TNF-a) kwa masiku a 2 kuti apeze ma DC omwe ali ndipamwamba kwambiri. kufotokoza kwa CD11c ndi CD83, omwe ali ndi mphamvu zolimbikitsa kuchulukitsa kwa maselo a allogeneic CD4 + T ndi CD8 + T. Ma polysaccharides ambiri ochokera kuzinthu zachilengedwe ali ndi ntchito yabwino kwambiri yoteteza thupi ku matenda, monga ma polysaccharides ochokera ku bowa wa shiitake, bowa wogawanika, bowa wa Yunzhi, ndi Poria cocos, zomwe zagwiritsidwa ntchito kuchipatala. Amatha kupititsa patsogolo chitetezo chamthupi, kulimbikitsa chitetezo chamthupi, komanso kukhala ngati ma adjuvant machiritso odana ndi chotupa. Komabe, pali malipoti ochepa ofufuza pa PFC ngati modulitsa chitetezo chamunthu. Chifukwa chake, nkhaniyi ikuchita kafukufuku woyambirira pazantchito ndi njira zofananira za PFC polimbikitsa kusasitsa kwa ma moDC, kuti awone kuthekera kwa PFC ngati modulator yachilengedwe ya chitetezo chamthupi.

Chifukwa cha kuchepa kwambiri kwa ma DC m'mitumbo ya anthu komanso kusungidwa kwamitundu yambiri pakati pa ma DC a mbewa ndi ma DC aumunthu, kuti athetse zovuta zofufuza zomwe zimayambitsidwa ndi kutsika kwa DC, ma in vitro induction model of DCs otengedwa m'maselo am'magazi amunthu. aphunziridwa, omwe angapeze ma DC ndi immunogenicity yabwino mu nthawi yochepa. Choncho, phunziroli linagwiritsa ntchito njira yachikhalidwe yopangira ma DCs aumunthu mu vitro: co culturing rhGM CSF ndi rhIL-4 in vitro, kusintha sing'anga tsiku lililonse, ndikupeza ma DCs osakhwima pa tsiku la 5th; Pa tsiku la 6th, mavoti ofanana a PBS, PFC, ndi LPS adawonjezeredwa malinga ndi magulu ndi chikhalidwe kwa maola a 24 monga chikhalidwe protocol chokopa ma DCs otengedwa ku maselo amtundu wamagazi a mononuclear aumunthu.

Ma polysaccharides opangidwa kuchokera kuzinthu zachilengedwe ali ndi ubwino wapoizoni wotsika mtengo komanso wotsika mtengo ngati ma immunostimulants. Pambuyo pakuyesa koyambirira, gulu lathu lofufuza lidapeza kuti PFC imakulitsa kwambiri cholembera chokhwima CD83 pamwamba pamagazi amunthu amtundu wa mononuclear amtundu wa DC omwe amapangidwa mu vitro. Zotsatira za cytometry zoyenda zimasonyeza kuti kulowererapo kwa PFC pamagulu a 10 μ g / mL kwa maola 24 kunapangitsa kuti pakhale chiwonetsero chapamwamba cha CD83 yokhwima pamwamba pa DCs, kusonyeza kuti DCs adalowa m'dziko lokhwima. Chifukwa chake, gulu lathu lofufuza lidatsimikiza za in vitro induction and intervention plan. CD83 ndi chinthu chofunikira kwambiri chokhwima pamwamba pa ma DC, pomwe CD86 imagwira ntchito ngati molekyulu yolimbikitsira pamwamba pa ma DC, kukhala ngati chizindikiro chachiwiri choyambitsa ma T cell. Mafotokozedwe owonjezereka a ma biomarkers awiri a CD83 ndi CD86 akuwonetsa kuti PFC imalimbikitsa kusasitsa kwa ma DC omwe amapangidwa ndi magazi amtundu wa mononuclear cell, kutanthauza kuti PFC ikhoza kuwonjezera nthawi yomweyo kuchuluka kwa ma cytokines pamwamba pa ma DC. Choncho, phunziroli linayesa milingo ya ma cytokines IL-6, TNF-a, ndi IL-10 opangidwa ndi DCs pogwiritsa ntchito ELISA. IL-10 imagwirizana kwambiri ndi kulekerera kwa chitetezo cha mthupi cha DCs, ndipo ma DC omwe ali ndi chitetezo chamthupi amagwiritsidwa ntchito kawirikawiri pochiza chotupa, kupereka malingaliro ochiritsira ochiritsira a chitetezo cha mthupi mu kupatsirana kwa ziwalo; Banja la 1L-6 limagwira ntchito yofunika kwambiri mu chitetezo chachibadwa komanso chosinthika, hematopoiesis, ndi zotsatira zotsutsana ndi kutupa; Pali maphunziro osonyeza kuti IL-6 ndi TGF β amatenga nawo mbali pakusiyanitsa kwa maselo a Th17; Thupi likagwidwa ndi kachilomboka, TNF-a yopangidwa ndi DCs poyankha ma virus activation imakhala ngati autocrine maturation factor kulimbikitsa kusasitsa kwa DC. Kuletsa TNF-a kudzayika ma DC mu siteji yachinyamata, kuwalepheretsa kuti asagwiritse ntchito mokwanira ntchito yawo yowonetsera antigen. Deta ya ELISA mu phunziroli inasonyeza kuti chiwerengero cha IL-10 mu gulu la PFC chinawonjezeka kwambiri poyerekeza ndi magulu ena awiri, kusonyeza kuti PFC imapangitsa kuti chitetezo cha mthupi chitetezeke ku DCs; Kuchulukirachulukira kwa IL-6 ndi TNF-a kukuwonetsa kuti PFC ikhoza kukhala ndi zotsatira zolimbikitsa DC kulimbikitsa kusiyanitsa kwa T cell.