M'magawo osiyanasiyana ofufuza ndi kugwiritsa ntchito ma microalgae, ukadaulo woteteza kwanthawi yayitali ma cell algae ndikofunikira. Njira zachikhalidwe zosungiramo algae zimakumana ndi zovuta zingapo, kuphatikizapo kuchepa kwa kukhazikika kwa majini, kuchuluka kwa ndalama, komanso kuwopsa kwa kuipitsa. Pofuna kuthana ndi mavutowa, protoga yapanga njira ya vitrification cryopreservation yoyenera ma microalgae osiyanasiyana. Kupanga njira yothetsera cryopreservation ndikofunikira kwambiri kuti mukhalebe wathanzi komanso kukhazikika kwa ma cell a microalgae.

Pakalipano, ngakhale kuti ntchito zopambana zakhala zikugwiritsidwa ntchito pa Chlamydomonas reinhardtii, kusiyana kwa thupi ndi ma cellular pakati pa mitundu yosiyanasiyana ya algae kumatanthauza kuti microalgae iliyonse ingafunike kupangidwa kwapadera kwa cryoprotectant. Poyerekeza ndi njira za cryopreservation zomwe zimagwiritsidwa ntchito m'njira zina zoteteza tizilombo toyambitsa matenda ndi nyama, njira yosungiramo tizilombo tating'onoting'ono tating'onoting'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'ono tating'onoting'ono timafunika kuganizira kamangidwe ka khoma la cell, kukana chisanu, komanso kuopsa kwa chitetezo cha ma cell a algae amitundu yosiyanasiyana.

Ukadaulo wa vitrification cryopreservation wa microalgae umagwiritsa ntchito njira zopangira ma cryopreservation kuti zisunge ma cell pamalo otsika kwambiri, monga nayitrogeni wamadzi kapena -80 ° C, pambuyo pa kuzizira kokhazikika. Makristalo a ayezi nthawi zambiri amapanga mkati mwa ma cell mkati mwa kuzizira, zomwe zimapangitsa kuwonongeka kwa ma cell ndikuwonongeka kwa maselo, zomwe zimapangitsa kuti maselo afe. Pofuna kupanga mayankho a microalgae cryopreservation, protoga idachita kafukufuku wozama pama cell a algae, kuphatikiza momwe amachitira ndi zoteteza zosiyanasiyana komanso momwe angachepetsere kuwonongeka komwe kumachitika chifukwa cha kuzizira komanso kuthamanga kwa osmotic. Izi zikuphatikiza kusintha kosalekeza kwa mtundu, kukhazikika, kutsatizana kowonjezera, kuziziritsa kusanachitike, ndi njira zochiritsira zoteteza mu njira ya cryopreservation, zomwe zimapangitsa kuti pakhale njira yotalikirapo ya microalgae cryopreservation solution yotchedwa Froznthrive ™ Ndi ukadaulo wothandizira kuzizira kwa vitrification.