Polysaccharide sitere na Chlorella (PFC), dị ka polysaccharide sitere n'okike, dọtara ọtụtụ nlebara anya site n'aka ndị ọkà mmụta n'afọ ndị na-adịbeghị anya n'ihi uru ya nke obere nsị, mmetụta dị ala, na mmetụta sara mbara. Ọrụ ya na iwetulata lipids ọbara, mgbochi tumor, mgbochi mkpali, mgbochi ọrịa Parkinson, mgbochi ịka nká, wdg bụ nke akwadoro na vitro na nnwale vivo. Otú ọ dị, a ka nwere oghere na nyocha na PFC dị ka onye na-ahụ maka mgbochi ọrịa mmadụ.

Selụ Dendritic (DCs) bụ sel ndị na-eweta antigen pụrụ iche kachasị ike na ahụ mmadụ. Ọnụ ọgụgụ nke DC n'ime ahụ mmadụ dị ntakịrị, na a na-ejikarị cytokine emezigharị na vitro induction model, ya bụ ọbara mmadụ mononuclear cell-derived DCs (moDCs), na-ejikarị. Ebu ụzọ kọwaa ụdị DC in vitro butere na 1992, nke bụ usoro ọdịnala ọdịnala maka DC. N'ozuzu, ọ chọrọ cultivation maka 6-7 ụbọchị. Enwere ike ịzụlite mkpụrụ ndụ ụmị ọkpụkpụ na granulocyte macrophage colony-stimulating factor (GM-CSF) na interleukin (IL) -4 iji nweta DCs akabeghị aka (PBS). A na-agbakwunye cytokines dị ka ihe mkpali tozuru okè ma mepụta ya maka ụbọchị 1-2 iji nweta DC tozuru okè. Nnyocha ọzọ kọrọ na sel CD14 + mmadụ dị ọcha ka ejiri interferon - β (IFN - β) ma ọ bụ IL-4 mee ihe maka ụbọchị 5, wee mepụta ya na tumor necrosis factor-a (TNF-a) maka ụbọchị 2 iji nweta DCs nwere elu. ngosipụta nke CD11c na CD83, nke nwere ikike siri ike iji kwalite mgbasa nke cell CD4 + T allogeneic na sel CD8 + T. Ọtụtụ polysaccharides sitere na isi mmalite nwere ezigbo ọrụ immunomodulatory, dị ka polysaccharides sitere na ero shiitake, mushrooms gbawara agbawa, mushrooms Yunzhi, na Poria cocos, bụ ndị etinyere n'ọrụ ụlọ ọgwụ. Ha nwere ike imeziwanye ọrụ ahụ ji alụso ọrịa ọgụ n'ụzọ dị irè, kwalite ihe mgbochi, ma jee ozi dị ka ọgwụgwọ adjuvant maka ọgwụgwọ mgbochi tumor. Agbanyeghị, enwere akụkọ nyocha ole na ole na PFC dị ka onye na-ahụ maka mgbochi ọrịa mmadụ. Ya mere, edemede a na-eme nyocha nke mbụ gbasara ọrụ na usoro ndị yiri ya nke PFC n'ịkwalite ntozu nke moDCs, iji nyochaa ike PFC dị ka onye na-ahụ maka nchebe anụ ahụ.

N'ihi oke dị ala nke DC na anụ ahụ mmadụ na nchekwa dị elu nke ụdị dị n'etiti òké DCs na DCs mmadụ, iji dozie nsogbu nyocha nke mmepụta DC dị ala kpatara, in vitro induction ụdị DC nke sitere na mkpụrụ ndụ mononuclear ọbara mmadụ. a mụọla ya, nke nwere ike nweta DC nwere ezigbo immunogenicity n'ime obere oge. Ya mere, ọmụmụ ihe a na-eji usoro ọdịnala na-eme ka mmadụ nwee DCs in vitro: co culturing rhGM CSF na rhIL-4 in vitro, na-agbanwe usoro ahụ kwa ụbọchị ọ bụla, na ịnweta DCs akabeghị aka na 5th ụbọchị; N'ụbọchị 6th, agbakwunyere nha nha nha nke PBS, PFC, na LPS dịka nchịkọta na omenala maka awa 24 dị ka usoro omenala maka ịkpalite DCs sitere na mkpụrụ ndụ mononuclear ọbara mmadụ.

Polysaccharides sitere na ngwaahịa eke nwere uru nke obere nsi na ọnụ ala dị ala dị ka immunostimulants. Ka emechara nnwale izizi, ndị otu nyocha anyị chọpụtara na PFC na-eme ka akara ngosi CD83 tozuru oke n'elu mkpụrụ ndụ DC sitere na mkpụrụ ndụ mononuclear cell na-ebute na vitro. Nsonaazụ cytometry na-efe efe gosiri na ntinye aka na PFC na ntinye nke 10 μ g/mL maka awa 24 butere nkwupụta kacha elu nke akara CD83 tozuru oke n'elu DC, na-egosi na DCs batara n'ọnọdụ tozuru oke. Ya mere, otu nyocha anyị kpebiri ntinye in vitro na atụmatụ ntinye aka. CD83 bụ ihe dị mkpa biomarker tozuru okè n'elu DCs, ebe CD86 na-eje ozi dị ka ihe mkpa co stimulatory molekul n'elu DCs, na-eme dị ka nke abụọ mgbaàmà maka ịgbalite T cell. Okwu nkwalite nke abụọ biomarkers CD83 na CD86 na-egosi na PFC na-akwalite maturation nke ọbara mmadụ mononuclear cell-ewepụtara DC, na-atụ aro na PFC nwere ike ịbawanye ọkwa nzuzo nke cytokines n'elu DC. Ya mere, ọmụmụ ihe a tụlere ọkwa nke cytokines IL-6, TNF-a, na IL-10 nke DC na-eji ELISA zoro ezo. IL-10 nwere njikọ chiri anya na mgbochi mgbochi nke DCs, na DC ndị nwere ndidi na-alụso ọrịa ọgụ na-ejikarị eme ihe na ọgwụgwọ tumor, na-enye echiche ndị nwere ike ịgwọ ọrịa maka ntachi obi na-alụso ọrịa ọgụ na transplantation akụkụ; Ezinụlọ 1L-6 na-arụ ọrụ dị mkpa na mgbochi ebumpụta ụwa na nke na-agbanwe agbanwe, hematopoiesis, na mmetụta mgbochi mkpali; Enwere ọmụmụ ihe na-egosi na IL-6 na TGF β jikọrọ aka na ọdịiche nke mkpụrụ ndụ Th17; Mgbe nje na-awakpo ahụ ahụ, TNF-a nke DC na-emepụta maka nzaghachi nje virus na-arụ ọrụ dị ka ihe na-eme ka autocrine maturation na-akwalite DC maturation. Igbochi TNF-a ga-etinye DCs n'ime oge akabeghị aka, na-egbochi ha ịrụ ọrụ ngosi antigen ha nke ọma. Ihe omuma nke ELISA n'ime ihe omumu a gosiputara na ọkwa nzuzo nke IL-10 n'ime ndi mmadu PFC bu nke oma ma e jiri ya tụnyere ndi ozo ndi ozo, na-egosi na PFC na-eme ka mgbochi nke DCs; Ọkwa nzuzo na-arịwanye elu nke IL-6 na TNF-a na-atụ aro na PFC nwere ike inwe mmetụta nke ịkwalite DC iji kwalite ọdịiche T cell.