N'akụkụ dị iche iche nke nyocha na ngwa microalgae, teknụzụ nke nchekwa ogologo oge nke mkpụrụ ndụ microalgae dị oke mkpa. Ụzọ nchekwa microalgae ọdịnala na-eche ọtụtụ ihe ịma aka ihu, gụnyere mbelata nkwụsi ike mkpụrụ ndụ ihe nketa, ọnụ ahịa ịrị elu, yana ihe egwu mmetọ na-abawanye. Iji lebara okwu ndị a anya, protoga ewepụtala usoro mkpuchi mkpuchi vitrification dabara adaba maka microalgae dị iche iche. Nhazi nke ngwọta cryopreservation dị oké mkpa maka ịnọgide na-enwe ike na nkwụsi ike mkpụrụ ndụ nke mkpụrụ ndụ microalgae.

Ka ọ dị ugbu a, ọ bụ ezie na etinyere ngwa na-aga nke ọma na Chlamydomonas reinhardtii, ọdịiche dị n'ụdị physiological na cellular dị n'etiti ụdị microalgae dị iche iche pụtara na microalgae ọ bụla nwere ike ịchọ usoro cryoprotectant. E jiri ya tụnyere ihe ngwọta cryopreservation eji na usoro microbial na anụmanụ cell cryopreservation, ngwọta cryopreservation maka microalgae kwesịrị ịtụle mgbidi cell, nguzogide ntu oyi, na mmeghachi omume na-egbu egbu nke ndị na-echebe mkpụrụ ndụ microalgae nke ụdị algal dị iche iche.

Teknụzụ vitrification cryopreservation nke microalgae na-eji ngwọta cryopreservation emebere nke ọma iji chekwaa mkpụrụ ndụ na oke okpomọkụ dị oke ala, dị ka nitrogen mmiri mmiri ma ọ bụ -80 Celsius C, mgbe emechara usoro jụrụ oyi. Kristal ice na-etolitekarị n'ime sel n'oge jụrụ oyi, na-emebi usoro cell na mfu nke ọrụ cell, na-eduga na ọnwụ cell. Iji mepụta ihe ngwọta microalgae cryopreservation, protoga mere nyocha miri emi na njirimara cellular nke microalgae, gụnyere mmeghachi omume ha na nchekwa dị iche iche yana otu esi ebelata mmebi nke oyi na nrụgide osmotic kpatara. Nke a na-agụnye mgbanwe na-aga n'ihu na ụdị, itinye uche, usoro mgbakwunye, tupu ịjụ oyi, na usoro mgbake nke ndị ọrụ nchebe na ngwọta cryopreservation, na-ebute mmepe nke nnukwu microalgae cryopreservation solution nke a na-akpọ Froznthrive ™ na teknụzụ na-akwado vitrification.