Polysaccharide los ntawm Chlorella (PFC), raws li ib tug natural polysaccharide, tau nyiam heev los ntawm cov kws tshawb fawb nyob rau hauv xyoo tas los no vim nws qhov zoo ntawm cov tshuaj toxicity tsawg, tsis tshua muaj tshwm sim, thiab broad-spectrum teebmeem. Nws ua haujlwm hauv kev txo cov ntshav lipids, tiv thaiv qog, tiv thaiv kab mob, tiv thaiv Parkinson's, tiv thaiv kev laus, thiab lwm yam tau raug lees paub ua ntej hauv vitro thiab hauv vivo kev sim. Txawm li cas los xij, tseem muaj qhov sib txawv hauv kev tshawb fawb ntawm PFC raws li tib neeg lub cev tiv thaiv kab mob.

Dendritic cells (DCs) yog lub zog tshwj xeeb tshaj plaws ntawm cov tshuaj tiv thaiv kab mob hauv tib neeg lub cev. Tus naj npawb ntawm DCs hauv tib neeg lub cev yog qhov tsawg heev, thiab ib qho cytokine mediated hauv vitro induction qauv, uas yog tib neeg peripheral ntshav mononuclear cell-derived DCs (moDCs), feem ntau yog siv. Tus qauv in vitro induced DC yog thawj zaug tshaj tawm hauv xyoo 1992, uas yog cov kab lis kev cai ib txwm muaj rau DCs. Feem ntau, nws yuav tsum tau cog qoob loo rau 6-7 hnub. Cov nas pob txha pob txha tuaj yeem raug coj los ua ke nrog granulocyte macrophage colony-stimulating factor (GM-CSF) thiab interleukin (IL) -4 kom tau txais DCs tsis paub qab hau (PBS pab pawg). Cytokines tau ntxiv raws li kev paub tab thiab kab lis kev cai rau 1-2 hnub kom tau txais DCs paub tab. Lwm txoj kev tshawb fawb tau tshaj tawm tias tib neeg CD14 + cov cell uas huv huv tau raug coj los ntawm interferon - β (IFN - β) lossis IL-4 rau 5 hnub, thiab tom qab ntawd coj cov qog necrosis factor-a (TNF-a) rau 2 hnub kom tau txais DCs nrog siab. Kev nthuav qhia ntawm CD11c thiab CD83, uas muaj peev xwm txhawb nqa kev loj hlob ntawm allogeneic CD4 + T hlwb thiab CD8 + T hlwb. Ntau polysaccharides los ntawm tej yam ntuj tso tej yam kev mob zoo heev immunomodulatory kev ua si, xws li polysaccharides los ntawm shiitake nceb, cais gill nceb, Yunzhi nceb, thiab Poria cocos, uas tau siv nyob rau hauv kev kho mob xyaum. Lawv tuaj yeem txhim kho lub cev tiv thaiv kab mob, txhim kho kev tiv thaiv kab mob, thiab ua cov kev kho mob ntxiv rau kev kho mob qog noj ntshav. Txawm li cas los xij, muaj qee qhov kev tshawb fawb qhia txog PFC raws li tib neeg lub cev tiv thaiv kab mob. Yog li, tsab xov xwm no ua kev tshawb fawb ua ntej ntawm lub luag haujlwm thiab cov txheej txheem cuam tshuam ntawm PFC hauv kev txhawb nqa kev loj hlob ntawm moDCs, txhawm rau ntsuas lub peev xwm ntawm PFC raws li lub cev tiv thaiv kab mob.

Vim qhov tsis tshua muaj kev faib ua feem ntawm DCs hauv tib neeg cov ntaub so ntswg thiab kev txuag kev sib txuam ntawm cov nas DCs thiab tib neeg DCs, txhawm rau daws cov teeb meem kev tshawb fawb los ntawm cov khoom siv DC tsawg, hauv vitro induction qauv ntawm DCs muab los ntawm tib neeg peripheral ntshav mononuclear hlwb. tau kawm, uas tuaj yeem tau txais DCs nrog kev tiv thaiv kab mob zoo hauv lub sijhawm luv luv. Yog li, txoj kev tshawb no siv cov txheej txheem ib txwm ua rau tib neeg DCs hauv vitro: co culturing rhGM CSF thiab rhIL-4 hauv vitro, hloov qhov nruab nrab txhua hnub, thiab tau txais DCs tsis paub qab hau rau hnub 5; Hnub tim 6, qhov sib npaug ntawm PBS, PFC, thiab LPS tau ntxiv raws li kev sib koom ua ke thiab kab lis kev cai rau 24 teev raws li kab lis kev cai raws cai rau inducing DCs tau los ntawm tib neeg peripheral ntshav mononuclear hlwb.

Polysaccharides muab tau los ntawm cov khoom ntuj tsim muaj qhov zoo ntawm cov tshuaj lom neeg tsawg thiab tus nqi qis raws li immunostimulants. Tom qab kev sim ua ntej, peb pab pawg tshawb fawb pom tias PFC tau txhim kho cov cim paub tab CD83 nyob rau saum npoo ntawm tib neeg peripheral ntshav mononuclear cell-derived DC hlwb induced hauv vitro. Flow cytometry cov txiaj ntsig tau pom tias PFC cuam tshuam ntawm qhov concentration ntawm 10 μ g / mL rau 24 teev ua rau lub siab tshaj plaws ntawm cov cim paub tab CD83 ntawm qhov chaw ntawm DCs, qhia tias DCs nkag mus rau hauv lub xeev laus. Yog li ntawd, peb pab pawg tshawb fawb tau txiav txim siab hauv vitro induction thiab cuam tshuam txoj kev npaj. CD83 yog ib qho tseem ceeb uas paub tab biomarker nyob rau saum npoo ntawm DCs, thaum CD86 yog ib qho tseem ceeb co stimulatory molecule nyob rau saum npoo ntawm DCs, ua raws li lub thib ob teeb liab rau activating T hlwb. Qhov kev nthuav qhia ntawm ob lub biomarkers CD83 thiab CD86 qhia tias PFC txhawb kev loj hlob ntawm tib neeg peripheral ntshav mononuclear cell-derived DCs, tawm tswv yim tias PFC yuav ib txhij nce qib secretion ntawm cytokines rau saum npoo ntawm DCs. Yog li, txoj kev tshawb no tau soj ntsuam cov qib ntawm cytokines IL-6, TNF-a, thiab IL-10 zais los ntawm DCs siv ELISA. IL-10 muaj feem xyuam nrog kev tiv thaiv kab mob ntawm DCs, thiab DCs nrog kev tiv thaiv kab mob feem ntau yog siv los kho qog, muab cov tswv yim kho mob rau kev tiv thaiv kab mob hauv lub cev; Tsev neeg 1L-6 plays lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob hauv lub cev thiab yoog raws, hematopoiesis, thiab tiv thaiv cov teebmeem; Muaj cov kev tshawb fawb qhia tias IL-6 thiab TGF β koom ua ke hauv kev sib txawv ntawm Th17 hlwb; Thaum lub cev raug cuam tshuam los ntawm tus kab mob, TNF-ib qho uas tsim los ntawm DCs teb rau tus kab mob ua kom ua rau muaj kev loj hlob ntawm autocrine los txhawb DC kev loj hlob. Thaiv TNF-a yuav muab DCs nyob rau hauv ib qho tseem ceeb theem, tiv thaiv lawv los ntawm tag nrho cov exerting lawv antigen kev nthuav qhia muaj nuj nqi. Cov ntaub ntawv ELISA hauv qhov kev tshawb fawb no tau pom tias qib kev tso tawm ntawm IL-10 hauv pawg PFC tau nce ntau dua piv rau lwm pawg, qhia tias PFC txhim kho kev tiv thaiv kab mob ntawm DCs; Cov qib secretion nce ntxiv ntawm IL-6 thiab TNF-a qhia tias PFC tuaj yeem muaj txiaj ntsig los txhawb DC los txhawb T cell sib txawv.