Hauv ntau qhov chaw ntawm microalgae kev tshawb fawb thiab kev siv, thev naus laus zis ntawm kev khaws cia ntev ntawm microalgae hlwb yog qhov tseem ceeb. Cov txheej txheem khaws cia microalgae ib txwm ntsib ntau yam teeb meem, suav nrog kev txo qis ntawm caj ces ruaj khov, nce nqi, thiab muaj kev phom sij ntau ntxiv. Txhawm rau daws cov teeb meem no, protoga tau tsim cov txheej txheem vitrification cryopreservation haum rau ntau yam microalgae. Lub formulation ntawm cov tshuaj cryopreservation yog ib qho tseem ceeb rau kev tswj cov vitality thiab genetic stability ntawm microalgae hlwb.

Tam sim no, txawm hais tias cov ntawv thov ua tiav tau ua tiav ntawm Chlamydomonas reinhardtii, qhov sib txawv ntawm lub cev thiab cov qauv ntawm tes ntawm cov microalgae sib txawv txhais tau hais tias txhua microalgae yuav xav tau cov txheej txheem cryoprotectant tshwj xeeb. Piv nrog rau cov kev daws teeb meem cryopreservation siv nyob rau hauv lwm yam microbial thiab tsiaj cell cryopreservation cov tswv yim, cov cryopreservation tov rau microalgae yuav tsum xav txog lub cell phab ntsa qauv, Frost tsis kam, thiab tshwj xeeb tshuaj tiv thaiv tiv thaiv rau microalgae hlwb ntawm txawv algal hom.

Lub vitrification cryopreservation technology ntawm microalgae siv tshwj xeeb tsim cov kev daws teeb meem cryopreservation los khaws cov hlwb ntawm qhov kub thiab txias, xws li kua nitrogen los yog -80 ° C, tom qab lub programmed txias txheej txheem. Cov dej khov nab kuab feem ntau tsim hauv cov hlwb thaum lub caij txias, ua rau kev puas tsuaj rau cov qauv ntawm tes thiab tsis ua haujlwm ntawm tes, ua rau cov cell tuag. Txhawm rau txhim kho microalgae cryopreservation cov kev daws teeb meem, protoga tau tshawb xyuas qhov tob ntawm cov yam ntxwv ntawm lub cev ntawm microalgae, suav nrog lawv cov kev cuam tshuam rau cov tshuaj tiv thaiv sib txawv thiab yuav ua li cas thiaj li txo tau qhov kev puas tsuaj los ntawm khov thiab osmotic siab. Qhov no suav nrog kev hloov kho tas mus li rau hom, kev xav, kev sib txuas ntxiv, ua ntej txias, thiab cov txheej txheem rov qab los ntawm cov tshuaj tiv thaiv hauv cov tshuaj cryopreservation, uas ua rau muaj kev loj hlob ntawm cov broad-spectrum microalgae cryopreservation tov hu ua Froznthrive ™ Thiab cov kev txhawb nqa vitrification freezing technology.