Polysaccharide daga Chlorella (PFC), a matsayin polysaccharide na halitta, ya ja hankalin masana da yawa a cikin 'yan shekarun nan saboda fa'idodinsa na ƙarancin guba, ƙarancin sakamako masu illa, da tasirin bakan. Ayyukansa na rage yawan lipids na jini, anti-tumor, anti-inflammatory, anti Parkinson's, anti-tsufa, da dai sauransu an riga an inganta su a cikin vitro da gwaje-gwajen vivo. Koyaya, har yanzu akwai tazara a cikin bincike akan PFC a matsayin mai daidaita garkuwar ɗan adam.

Kwayoyin Dendritic (DCs) sune mafi ƙarfi na musamman na sel masu gabatar da antigen a cikin jikin ɗan adam. Adadin DCs a cikin jikin ɗan adam yana da ƙanƙanta sosai, kuma cytokine da aka yi sulhu a cikin ƙirar induction in vitro, wato ɗan adam na gefe na jini mononuclear cell-derived DCs (moDCs), ana amfani da su akai-akai. An fara ba da rahoton samfurin in vitro na DC a cikin 1992, wanda shine tsarin al'adun gargajiya na DCs. Gabaɗaya, yana buƙatar namo don kwanaki 6-7. Kwayoyin kasusuwan kasusuwa na linzamin kwamfuta za a iya al'ada tare da granulocyte macrophage colony-stimulating factor (GM-CSF) da interleukin (IL) -4 don samun DCs marasa girma (PBS). Ana ƙara cytokines a matsayin manyan abubuwan motsa jiki da kuma al'ada na kwanaki 1-2 don samun manyan DCs. Wani binciken ya ba da rahoton cewa an haɓaka ƙwayoyin CD14 + na ɗan adam mai tsabta tare da interferon - β (IFN - β) ko IL-4 don kwanaki 5, sannan kuma an tsara su tare da ƙwayar cutar necrosis factor-a (TNF-a) don kwanaki 2 don samun DCs tare da girma. Maganar CD11c da CD83, waɗanda ke da ƙarfin ƙarfi don inganta haɓakar ƙwayoyin CD4 + T da CD8 + T. Yawancin polysaccharides daga tushen halitta suna da kyakkyawan aiki na rigakafi, irin su polysaccharides daga namomin kaza na shiitake, namomin kaza da aka raba, namomin Yunzhi, da Poria cocos, waɗanda aka yi amfani da su a aikin asibiti. Suna iya inganta aikin garkuwar jiki yadda ya kamata, haɓaka rigakafi, da kuma zama hanyoyin kwantar da hankali don maganin ƙwayar cuta. Koyaya, akwai ƴan rahotannin bincike akan PFC a matsayin mai modulator na rigakafi na ɗan adam. Sabili da haka, wannan labarin yana gudanar da bincike na farko kan rawar da hanyoyin da suka danganci PFC wajen haɓaka balaga na moDCs, don kimanta yuwuwar PFC a matsayin mai daidaita yanayin rigakafi na halitta.

Saboda ƙarancin ƙarancin DCs a cikin kyallen jikin ɗan adam da babban kiyaye nau'ikan nau'ikan tsakanin linzamin kwamfuta da DCs, don magance matsalolin bincike da ke haifar da ƙarancin samar da DC, in vitro induction model na DCs waɗanda aka samo daga sel mononuclear na jikin ɗan adam. An yi nazari, wanda zai iya samun DCs tare da ingantaccen rigakafi a cikin ɗan gajeren lokaci. Sabili da haka, wannan binciken ya yi amfani da hanyar gargajiya na haifar da DCs a cikin vitro: co culturing rhGM CSF da rhIL-4 in vitro, canza matsakaici a kowace rana, da samun DCs marasa girma a ranar 5th; A rana ta 6, an ƙara daidai adadin PBS, PFC, da LPS bisa ga haɗawa da al'ada na sa'o'i 24 a matsayin ƙa'idar al'ada don ƙaddamar da DCs da aka samo daga sel mononuclear na ɗan adam.

Polysaccharides da aka samo daga samfuran halitta suna da fa'idodin ƙarancin guba da ƙarancin farashi azaman immunostimulants. Bayan gwaje-gwaje na farko, ƙungiyar bincikenmu ta gano cewa PFC tana haɓaka babban alamar CD83 akan saman jikin ɗan adam na jikin ɗan adam wanda aka samu tantanin halitta DC wanda aka haifar a cikin vitro. Sakamakon cytometry na gudana ya nuna cewa shiga tsakani na PFC a matakin 10 μ g/mL na tsawon awanni 24 ya haifar da mafi girman magana na babban alamar CD83 a saman DCs, yana nuna cewa DCs sun shiga yanayin balagagge. Don haka, ƙungiyar binciken mu ta ƙaddara tsarin shigar da in vitro da shirin shiga tsakani. CD83 shine mahimmin balagagge mai ƙima a saman DCs, yayin da CD86 ke aiki a matsayin muhimmin ƙwayar ƙwayar cuta mai haɓakawa a saman DCs, yana aiki azaman sigina na biyu don kunna ƙwayoyin T. Ingantattun maganganun CD83 da CD86 guda biyu suna nuna cewa PFC yana haɓaka maturation na ɗan adam tantanin halitta da aka samu DCs, yana nuna cewa PFC na iya haɓaka matakin ɓoyewar cytokines a saman DCs. Saboda haka, wannan binciken ya kimanta matakan cytokines IL-6, TNF-a, da IL-10 da aka ɓoye ta DCs ta amfani da ELISA. IL-10 yana da alaƙa da haɗin kai na rigakafi na DCs, kuma DCs tare da juriya na rigakafi ana amfani da su akai-akai a cikin maganin ƙwayar cuta, suna ba da ra'ayoyin warkewa mai yuwuwa don jurewar rigakafi a cikin dashen gabobin; Iyalin 1L-6 suna taka muhimmiyar rawa a cikin rigakafi na asali da kuma daidaitawa, hematopoiesis, da cututtukan cututtuka; Akwai nazarin da ke nuna cewa IL-6 da TGF β tare suna shiga cikin bambance-bambancen ƙwayoyin Th17; Lokacin da kwayar cutar ta mamaye jiki, TNF-a wanda DCs ke samarwa don amsawa ga kunnawar ƙwayar cuta yana aiki azaman nau'in maturation na autocrine don haɓaka maturation na DC. Katange TNF-a zai sanya DCs a cikin matakin da bai balaga ba, yana hana su cikakken aiwatar da aikin gabatar da antigen. Bayanan ELISA a cikin wannan binciken ya nuna cewa matakin ɓoye na IL-10 a cikin ƙungiyar PFC ya karu sosai idan aka kwatanta da sauran kungiyoyi guda biyu, yana nuna cewa PFC yana inganta haɓakar rigakafi na DCs; Ƙara yawan matakan ɓoye na IL-6 da TNF-a suna ba da shawarar cewa PFC na iya samun tasirin haɓaka DC don inganta bambancin ƙwayoyin T.